Table 1 Protein concentration was determined after dissolving the pellets in the re-swelling buffer; parameters are presented as the mean ± for triplicates
Extraction method | Number of spots | Protein concentration μg/μl |
|---|---|---|
PEG fractionation method | 750 | 8.9 ± 0.07 |
Phenol extraction method | 525 | 4.6 ± 0.08 |
TCA–acetone method | 110 | 2.5 ± 0.005 |
Phenol and TCA–acetone method | 231 | 3.9 ± 0.003 |
Imidazole method | Not detected | 1.6 ± 0.02 |