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Table 5 Detoxification enzyme activities after P. ribesioides extracts were sprayed on 3rd instar S. exigua larvae in greenhouse after 24 h exposure

From: Antifeedant Activity and Biochemical Responses in Spodoptera exigua Hübner (Lepidoptera: Noctuidae) Infesting Broccoli, Brassica oleracea var. alboglabra exposed to Piper ribesioides Wall Extracts and Allelochemicals

Compounds

Carboxylesterase

activityA

CFB

Glutathione-S-transferase activityA

CFB

Acetylcholinesterase

activityA

CFB

Control

0.062 ± 0.013bc

0.184 ± 0.003bc

0.107 ± 0.003a

Hexane extract

0.032 ± 0.004a

1.94

0.163 ± 0.006b

1.13

0.092 ± 0.005a

1.16

Ethyl acetate extract

0.055 ± 0.007b

1.13

0.141 ± 0.008a

1.30

0.088 ± 0.012a

1.22

Methanol extract

0.077 ± 0.004c

0.81

0.176 ± 0.003bc

1.05

0.197 ± 0.015c

0.54

Cypermethrin

0.107 ± 0.005d

0.58

0.195 ± 0.004c

0.94

0.144 ± 0.008b

0.74

P-value

 < 0.0001

 < 0.0001

 < 0.0001

F-value

43.947

52.880

65.368

  1. AMean values within a column followed by same letter were not significantly different (P < 0.05) by Tukey’s test. Carboxylesterase (CE) measured as p-nitrophenol/min/mg esterase in nM. Glutathione-S-transferase (GST) measured as glutathione conjugated product/min/mg in nM. Acetylcholinesterase (AChE) measured as µM/min/mg of protein
  2. BCorrection factor (CF) of detoxification enzyme activities calculated as enzyme activity in controls / enzyme activity in treatments