The use of osmoregulators and antioxidants to mitigate the adverse impacts of salinity stress in diploid and tetraploid potato genotypes (Solanum spp.)

Zaki, Haitham E. M.; Radwan, Khlode S. A.

doi:10.1186/s40538-022-00286-3

Chemical and Biological Technologies in Agriculture

Table 5 Salinity tolerance index (STI) of 13 genotypes of potato divided into 7 tetraploid cultivars of Solanum tuberosum (tbr), and 6 diploid clones of S. chacoense (chc) grown in vitro under conditions of high salt stress level (300 mmol L⁻¹ NaCl) with and without glycine betaine (at 400 mg L⁻¹), proline (at 200 mg L⁻¹) or salicylic acid (at 100 mg L⁻¹) treatments

From: The use of osmoregulators and antioxidants to mitigate the adverse impacts of salinity stress in diploid and tetraploid potato genotypes (Solanum spp.)

Traits	S. tuberosum (tbr)							S. chacoense (chc)
Traits	Agata	Atlantic	Anya	Diamond	Gala	Kennebec	Russet Burbank	A-6	B-7	C-8	C-9	D-2	D-3
STI under high stress
Roots fresh weight (g)	0.45	0.23	0.102	0.66	0.12	0.42	0.61	0.95	0.52	0.98	0.38	0.58	0.91
Surface area of root (cm²)	0.43	0.45	0.20	0.71	0.12	0.49	0.58	0.73	0.43	0.99	0.75	0.70	0.53
Total root length (mm)	0.89	0.61	0.32	0.80	0.15	0.74	0.75	0.86	0.32	0.86	0.36	0.89	0.93
No. of roots/plantlet	0.59	0.77	0.15	0.81	0.11	0.87	0.70	0.84	0.70	0.85	0.58	0.86	0.63
Plantlet water content%	0.77	0.70	0.43	0.83	0.39	0.70	0.70	0.94	0.66	0.94	0.69	0.91	0.76
Chloride (mmol kg⁻¹ FW)	9.13	8.32	11.37	8.18	11.53	10.86	5.45	10.50	12.50	12.78	8.51	11.52	13.64
Sodium (mmol kg⁻¹ FW)	9.21	9.07	10.84	8.03	10.70	12.23	5.75	17.00	18.43	11.93	13.46	12.09	18.90
Potassium (mmol kg⁻¹ FW)	0.37	0.37	0.28	0.47	0.26	0.38	0.43	0.70	0.34	0.95	0.54	0.84	0.73
Chlorophyll (SPAD)	0.52	0.57	0.33	0.76	0.33	0.51	0.59	0.83	0.44	0.84	0.61	0.84	0.66
Catalase (U min⁻¹ g⁻¹ FW)	3.11	2.95	3.28	3.01	3.31	3.26	2.94	1.77	2.83	1.46	3.45	1.68	3.62
Peroxidase (U min⁻¹ g⁻¹ FW)	3.08	3.11	3.55	2.89	3.66	3.44	3.13	1.567	2.880	1.333	3.175	1.667	3.154
Callus fresh weight (g)	0.27	0.24	0.00	0.79	0.00	0.34	0.20	1.09	0.46	0.91	0.63	0.77	0.90
Explants forming callus %	0.34	0.48	0.00	0.93	0.00	0.37	0.41	0.97	0.70	1.02	0.37	0.77	0.98
Explants forming roots%	0.00	0.00	0.00	0.49	0.00	0.01	0.00	0.91	0.91	0.93	0.72	0.65	0.82
STI under high stress + proline
Roots fresh weight (g)	0.94	0.49	0.102	1.07	0.09	0.55	0.97	1.37	1.45	1.41	1.23	1.04	1.48
Surface area of root (cm²)	0.85	0.90	0.44	0.88	0.35	0.91	0.69	0.78	0.61	0.99	0.81	0.82	0.65
Total root length (mm)	0.91	0.73	0.50	0.85	0.38	0.85	0.79	0.91	0.88	0.91	0.80	0.96	0.96
No. of roots/plantlet	0.94	0.88	0.67	0.96	0.48	0.91	0.95	0.98	0.94	0.97	0.87	0.94	0.91
Plantlet water content%	0.89	0.81	0.60	0.95	0.54	0.80	0.81	0.83	0.80	0.90	0.74	0.87	0.82
Chloride (mmol kg⁻¹ FW)	3.85	3.66	4.06	3.50	3.96	4.31	2.27	7.52	5.85	9.15	3.95	5.21	6.43
Sodium (mmol kg⁻¹ FW)	4.09	4.21	4.03	4.88	4.07	5.80	3.18	8.22	6.46	8.57	4.53	5.46	6.39
Potassium (mmol kg⁻¹ FW)	0.43	0.36	0.32	0.45	0.31	0.28	0.47	0.67	0.52	0.73	0.43	0.39	0.44
Chlorophyll (SPAD)	0.73	0.78	0.65	0.88	0.65	0.63	0.78	0.87	0.73	0.89	0.80	0.86	0.84
Catalase (U min⁻¹ g⁻¹ FW)	1.82	1.22	1.87	1.05	1.80	1.85	1.32	1.49	2.14	1.53	1.90	1.47	1.99
Peroxidase (U min⁻¹ g⁻¹ FW)	1.86	2.05	2.18	1.41	2.28	1.76	1.30	1.42	2.00	1.33	1.59	1.29	1.54
Callus fresh weight (g)	0.62	0.82	0.26	0.85	0.29	0.84	0.71	1.11	0.81	0.98	0.82	0.82	0.94
Explants forming callus %	0.90	0.92	0.76	0.95	0.77	0.83	0.87	0.99	0.89	1.03	0.92	0.93	0.99
Explants forming roots%	0.46	0.45	0.51	0.71	0.52	0.47	0.70	0.96	0.92	0.96	0.83	0.75	0.86
STI under high stress + salicylic acid
Roots fresh weight (g)	1.31	0.49	0.142	1.28	0.13	0.96	1.25	1.57	1.63	1.56	1.42	1.12	1.70
Surface area of root (cm²)	0.87	0.91	0.65	0.89	0.45	0.93	0.85	0.84	0.88	1.10	0.87	0.92	0.79
Total root length (mm)	0.95	0.84	0.64	0.96	0.49	0.91	0.90	0.96	0.98	0.96	0.96	1.01	1.02
No. of roots/plantlet	0.99	1.01	0.86	1.00	0.71	0.96	0.98	1.00	0.97	0.98	0.92	0.98	0.93
Plantlet water content%	0.93	0.82	0.77	0.95	0.73	0.86	0.82	0.95	0.86	0.96	0.87	0.92	0.89
Chloride (mmol kg⁻¹ FW)	2.82	2.75	3.13	2.32	3.03	3.17	1.55	2.03	2.14	1.98	1.78	2.22	2.24
Sodium (mmol kg⁻¹ FW)	3.48	3.67	3.80	3.76	3.81	5.47	2.53	2.32	1.77	2.37	1.16	1.29	1.74
Potassium (mmol kg⁻¹ FW)	0.74	0.72	0.56	0.83	0.56	0.48	0.86	0.91	0.81	0.87	0.81	0.76	0.79
Chlorophyll (SPAD)	0.80	0.84	0.75	0.92	0.76	0.77	0.87	0.96	0.83	0.98	0.86	0.92	0.90
Catalase (U min⁻¹ g⁻¹ FW)	1.59	1.08	1.57	1.05	1.58	1.63	1.18	1.77	2.00	1.46	2.18	1.47	2.06
Peroxidase (U min⁻¹ g⁻¹ FW)	1.32	1.44	2.34	1.56	2.39	1.44	1.60	1.34	1.68	1.26	1.59	1.21	1.46
Callus fresh weight (g)	0.76	0.94	0.41	0.93	0.50	0.96	0.88	1.14	1.01	1.08	0.99	0.86	1.03
Explants forming callus %	0.94	0.94	0.86	0.99	0.89	0.92	0.93	1.01	0.97	1.03	0.95	0.97	1.02
Explants forming roots%	0.54	0.56	0.62	0.80	0.67	0.59	0.73	0.98	0.96	0.97	0.92	0.83	0.90
STI under high stress + glycine betaine
Roots fresh weight (g)	1.43	1.02	0.36	1.54	0.50	1.02	1.55	1.93	2.01	2.02	1.95	1.52	2.07
Surface area of root (cm²)	0.96	0.96	0.84	0.99	0.55	0.96	0.91	0.97	0.94	1.17	0.91	0.96	0.90
Total root length (mm)	0.95	0.84	0.64	0.96	0.49	0.91	0.90	1.05	0.99	1.02	0.98	1.02	1.05
No. of roots/plantlet	1.03	1.06	0.93	1.02	0.77	1.01	1.01	1.11	1.01	1.00	0.94	1.16	0.95
Plantlet water content%	0.97	0.83	0.81	0.97	0.75	0.90	0.84	1.00	0.95	1.00	0.96	0.98	0.99
Chloride (mmol kg⁻¹ FW)	1.43	1.40	2.43	1.04	2.21	1.75	1.02	1.28	1.32	1.26	1.13	0.96	1.30
Sodium (mmol kg⁻¹ FW)	1.16	1.59	2.24	1.14	2.22	1.67	0.97	1.38	1.24	1.21	1.06	1.25	1.23
Potassium (mmol kg⁻¹ FW)	0.92	0.88	0.81	0.97	0.79	0.71	1.03	0.91	0.96	0.96	0.88	0.79	0.83
Chlorophyll (SPAD)	0.98	0.94	0.86	0.99	0.91	0.91	0.98	0.99	0.93	0.99	0.97	0.99	0.97
Catalase (U min⁻¹ g⁻¹ FW)	1.59	1.37	1.57	0.83	1.58	1.70	1.25	0.92	1.93	1.18	2.04	1.26	1.91
Peroxidase (U min⁻¹ g⁻¹ FW)	1.24	1.29	2.02	1.04	2.11	1.52	1.45	1.27	1.60	1.26	1.43	1.14	1.38
Callus fresh weight (g)	0.93	0.99	0.66	0.98	0.70	0.99	0.97	1.21	1.10	1.23	1.26	1.04	1.08
Explants forming callus %	0.98	0.99	0.93	1.01	0.95	0.97	0.96	1.03	1.01	1.04	1.01	1.01	1.03
Explants forming roots%	0.71	0.80	0.78	0.86	0.81	0.81	0.81	1.01	0.99	1.01	0.95	0.88	0.94

Back to article page