Fig. 4

Comparison of the resolution of CBB staining methods for rice endosperm total cell protein extracts. A Comparison of the protein profiles of gels stained using the three methods. The gel stained using the improved colloidal CBB-G staining shows sharper and better separation of proteins bands. B The ImageJ plot profiles for the lanes loaded with 20.0 µg total proteins as indicated by the rectangular boxes. The sections circled and labelled H, M, and L are the high, medium, and low molecular weight sections. C Close-up view of the high, medium, and low molecular weight sections. The arrows indicate the peak differences that can be observed. The improved colloidal CBB-G staining method plot profile shows peaks which are sharper and more distinct, indicating improved protein band resolution