Protease-producing lactic acid bacteria with antibacterial properties and their potential use in soybean meal fermentation

Chemical and Biological Technologies in Agriculture

Table 2 Physiological and biochemical analysis of protease-producing lactic acid bacteria strains

Isolates	Growth at temperature (°C)				Growth in NaCl (w/v, %)		Growth at pH
Isolates	4.0	10.0	45.0	50.0	3.0	6.5	3.0	3.5	4.0	4.5	8.0	9.0	10.0
P3	−	+	w	−	−	−	w	−	+	+	+	w	−
P8	−	+	w	w	+	−	−	−	+	+ +	+	w	−
P12	−	+	+	−	+	w	w	+	+	+	+	+	−
P13	−	+	+	+	+	+	+	+	+	+ +	+	+	+
P14	−	+	+	w	+	w	−	+	+	+ +	+	w	−
P15	+	+ +	+	+	+	+	+	+	+	+ +	+	+	+
P20	−	+	−	−	−	−	−	−	+	+	+	+	w
P22	−	+	−	−	+	−	−	−	+	+	+	+	+
P24	+	+	+	+	+	+	+	+	+	+ +	+ +	+	+
ZZUPF92	−	+ +	+	−	w	+	+	+	+	+	+	+	+
ZZUPF94	+	+ +	+	+	+	+	+	+	+	+ +	+	+	+
ZZUPF95	+	+ +	+	+	+	+	+	+	+	+ +	+	+	+
ZZUPF145	−	+	+	w	w	−	w	w	+	+	+	+	+
LAO49	+	+	+	+	+	+	+	+	+	+	+	+	+
LAO53	+	+	+ +	+	+	+	+	+	+	+ +	+	+	+
LAO56	+	+	+	+	+	+	+	+	+	+	+	+	+
LAO60	−	+	+	+	+	+	−	w	+	+	+	+	w
LAO66	−	+	+	w	+	−	w	+	+	+	+	−	−
MB56	−	+	+	+	+	−	−	+	+	+	+	+	−
MB66	+	+	+	+	+	+	+	+	+	+	+	+	+
MB72	−	+	+	+	+	w	+	+	+	+	+	−	−
MB73	−	+	+	w	+	w	−	+	+	+	+	−	−
MB76	+	+	+	w	+	w	−	+	+	+	+	+	−

All representative strains were positive for Gram stain and negative for catalase reaction
For fermentation type, all representative strains are homofermentative except LAO56, which was heterofermentative
+ + grow well; + could grow; − could not grow; w could weakly grow