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Chemical and Biological Technologies in Agriculture
Fig. 1 | Chemical and Biological Technologies in Agriculture

Fig. 1

From: Improved CTAB method for RNA extraction of thick waxy leaf tissues from sago palm (Metroxylon sagu Rottb.)

Fig. 1

TBE-AGE assay of sago palm leaf tissue RNA extract between improved CTAB method, PCI method and MRIP methods. Line M = GeneRuler 1 kb DNA Ladder, lane 1 = improved CTAB method replicate 1, lane 2 = improved CTAB method replicate 2, lane 3 = PCI method replicate 1, lane 4 = PCI method replicate 2, lane 5 = MRIP method replicate 1, lane 6 = MRIP method replicate 2. Improved CTAB method shows the best results as shown by the present of intact 28 s and 18 s rRNA band. There is presence of smearing at the 5 s region for CTAB method and PCI method but more significant in the latter method. No band is observed in MRIP method

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