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Table 8 RNA sequencing clean reads quality metrics from sequencing report.

From: Improved CTAB method for RNA extraction of thick waxy leaf tissues from sago palm (Metroxylon sagu Rottb.)

Sample

Total raw reads (Mb)

Total clean reads (Mb)

Total clean bases (Gb)

Clean reads Q20 (%)

Clean reads Q30 (%)

Clean reads ratio (%)

T1

69.96

66.82

6.68

97.88

91.45

95.51

T2

69.96

66.97

6.7

98.09

92.07

95.72

T3

69.96

66.68

6.67

97.76

91.14

95.31

N1

69.96

66.66

6.67

98

91.78

95.27

N2

69.41

66.62

6.66

97.94

91.59

95.99

N3

69.96

67.33

6.73

97.98

91.63

96.24

  1. All the samples show good sequencing results with above average clean read ratio and clean read Q30 ratio
  2. Sample: T1, T2, T3, N1, N2, and N3 are biological replicates of mature sago palm leaf RNA extract
  3. Total raw reads (Mb): the reads amount before filtering
  4. Total clean reads (Mb): the reads amount after filtering
  5. Total clean bases (Gb): the total base amount after filtering
  6. Clean reads Q20 (%): the rate of bases which quality is greater than 20 value in clean reads
  7. Clean reads Q30 (%): the rate of bases which quality is greater than 30 value in clean reads
  8. Clean reads ratio (%): the ratio of the amount of clean reads