Sample | Total number | Total length | Mean length | N50 | N70 | N90 | GC (%) |
|---|
T1 | 75,730 | 56,765,699 | 749 | 1334 | 740 | 283 | 45.54 |
T2 | 94,989 | 73,822,623 | 777 | 1447 | 778 | 285 | 44.11 |
T3 | 84,578 | 65,560,398 | 775 | 1430 | 776 | 285 | 44.67 |
N1 | 68,068 | 55,890,222 | 821 | 1472 | 860 | 309 | 45.44 |
N2 | 91,100 | 65,484,623 | 718 | 1303 | 684 | 270 | 44.75 |
N3 | 97,205 | 78,047,560 | 802 | 1503 | 821 | 293 | 43.65 |
- All the samples show similar assembly results with mean length above 700 bp and N50 above 1300
- Sample: T1, T2, T3, N1, N2, and N3 are biological replicates of mature sago palm leaf RNA extract
- Total number: the total number of transcripts
- Total length: the read length of transcripts
- Mean length: the average length of transcripts
- N50: the N50 length is used to determine the assembly continuity, the higher the better. N50 is a weighted median statistic that 50% of the total length is contained in Unigenes that are equal to or larger than this value;
- N70: similar to N50
- N90: similar to N50
- GC (%): the percentage of G and C bases in all transcripts
