Fig. 7

pH (A) and temperature (B) curves of cellulase enzyme in the cell homogenate fraction of strain CV2-1. The values were expressed as Relative Activity (%). Buffer solutions employed: Glycine pH 3 (G3), Acetate pH 3.5 (A3.5), Acetate pH 4 (A4), Acetate pH 4.6 (A4.6), Acetate pH 5 (A5), Acetate pH 5.6 (A5.6), Phosphate pH 6 (P6); Phosphate pH 6.5 (P6.5), Phosphate pH 7 (P7), Phosphate pH 8 (P8), Phosphate pH 8.6 (P8.6), Glycine pH 9 (G9) and pH 10 (G10). The values after 1 h of incubation were expressed as relative activity (%) and they were subtracted from the contribution by the single sample and substrate at the time zero. The reported values are an average of three replicates and bars are the corresponding standard deviations