Polysaccharides from Dioscorea opposita Thunb.: isolation, structural characterization, and anti-inflammatory and anti-tumor effects against hepatocellular carcinoma

Chemical and Biological Technologies in Agriculture

Table 2 Methylation analysis of DOP1-1 and DOP2-1

Methylation product	Mass fragments (m/z)	Molar ratio (100%)	Type of linkages
DOP1-1
2,3,6-Me₃-Glc	43, 45, 71, 87, 99, 101, 113, 117, 129, 161, 233	5.3	→ 4) Glcp (1 →
1,2,5-Ac₃-3,4,6-Me₃-D-Man	71, 129, 161	63.2	→ 2) Manp (1 →
2,3,6-Me₃-Gal	43, 71, 87, 99, 101, 113, 118, 129, 131, 145, 161, 174, 233	26.7	→ 4) Galp (1 →
DOP2-1
3,4-Me₂-Rha	43, 45, 58, 60, 71, 73, 87, 101, 110, 117, 128	1.7	→ 2) Rhap (1 →
2,3,6-Me₃-Glc	43, 45, 71, 87, 99, 101, 113, 117, 129, 161, 233	17.8	→ 4) Glcp (1 →
1,4,5-Ac₃-2,3,6-Me₃-D-Man	71, 87, 99, 101, 113, 117, 129, 161, 173, 233	27.1	→ 4) Manp (1 →
1,4,5-Ac₃-2,3-Me₃-D-Ara	71, 87, 101, 117, 129, 161, 189	5.3	→ 5) Araf (1 →
1,3,5,6-Ac₄-2,4-Me₂-D-Man	71, 87, 101, 117, 129, 189, 233	7.4	→ 3,6) Manp (1 →
2,3,6-Me₃-Gal	43, 71, 87, 99, 101, 113, 118, 129, 131, 145, 161, 174, 233	32.3	→ 4) Galp (1 →