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Chemical and Biological Technologies in Agriculture

Table 4 Fermentation quality and microbial composition of stylo silagesa

From: Fermentation quality and bacterial community of delayed filling stylo silage in response to inoculating lactic acid bacteria strains and inoculating time

Treatmentsb

 

DM

pH

Organic acid (g/kg DM)

NH3–N

Microorganisms (lg cfu/g FM)

  

(g/kg FM)

 

Lactic acid

Acetic acid

Propionic acid

Butyric acid

(g/kg TN)

LAB

Aerobic bacteria

Quick filling

CK

286.7i

4.98a

10.46 h

11.98a

1.40a

2.57ab

178.73a

6.20

5.07e

 

SXC48

296.7hi

4.72def

21.65def

9.00b

0.08 fg

0.81ab

122.57e

6.43

5.19e

 

CCZZ1

297.1hi

4.63 fg

19.81ef

8.02bc

0.03 g

0.99ab

124.59e

6.37

5.05e

 

XC124

298.9hi

4.88b

12.12gh

9.14b

0.23defg

2.13ab

158.49b

6.31

5.13e

Delayed filling (1 d)

CK

312.7fgh

5.01a

14.36gh

9.46b

0.62bc

2.74ab

160.67b

6.27

6.35bcd

Inoculating at chopping

SXC48

307.6gh

5.00a

14.42gh

9.55b

0.74b

2.74ab

147.20bcd

6.11

6.13 cd

 

CCZZ1

321.9efg

4.72def

19.60efg

7.65bc

0.17defg

0.54b

140.35bcde

6.10

5.93d

 

XC124

327.0cdef

4.80bcd

22.07def

9.23b

0.43cde

0.74ab

141.92bcde

6.07

5.94d

Inoculating at filling

SXC48

322.5efg

4.71def

16.90fgh

6.32c

0.19defg

0.70ab

150.10bcd

5.90

6.17 cd

 

CCZZ1

321.6efg

4.69ef

23.32def

8.35bc

0.20defg

0.60b

155.92bc

6.33

6.12 cd

 

XC124

324.6def

4.78cde

21.65def

8.83bc

0.48bcd

0.67b

136.67cde

6.36

6.09 cd

Delayed filling (2 d)

CK

341.8bc

4.73def

30.89bc

7.58bc

0.58bc

2.41ab

133.96de

6.46

6.64abc

Inoculating at chopping

SXC48

328.3cdef

4.84bc

24.21de

7.02bc

0.38cdef

3.03a

131.18de

6.43

7.11a

 

CCZZ1

356.5ab

4.47i

33.21bc

7.25bc

0.11efg

0.60b

141.02bcde

5.73

6.84ab

 

XC124

363.2a

4.58gh

28.30 cd

7.27bc

0.26defg

1.34ab

142.24bcde

6.27

6.59abc

Inoculating at filling

SXC48

334.3cde

4.42i

40.81a

8.27bc

0.16efg

1.07ab

150.55bcd

5.92

6.45bcd

 

CCZZ1

334.0cde

4.44i

32.70bc

7.01bc

0.14efg

1.00ab

145.62bcd

5.59

6.58abc

 

XC124

340.9bcd

4.51hi

37.10ab

7.20bc

0.26defg

1.87ab

151.43bcd

5.53

6.52abcd

SEMc

 

1.234

0.008

0.502

0.178

0.023

0.162

1.481

0.072

0.044

Significanced

D

**

**

**

**

**

NS

NS

NS

**

 

I

**

**

*

**

**

NS

**

NS

NS

 

T

NS

**

**

NS

NS

NS

NS

NS

NS

 

D × I

NS

NS

NS

NS

NS

NS

*

NS

NS

 

D × T

**

NS

*

NS

NS

NS

NS

NS

*

 

I × T

**

**

*

NS

**

*

NS

NS

NS

 

D × I × T

NS

NS

*

*

NS

NS

NS

NS

NS

  1. Different lowercase letters in the same column indicate significant difference (P < 0.05)
  2. aDM, dry matter; FM, fresh matter; NH3–N, ammonia nitrogen; TN, total nitrogen; LAB, lactic acid bacteria
  3. bCK, without inoculation; SXC48 and CCZZ1, inoculating Lb. plantarum SXC48 and CCZZ1; XC124, inoculating En. faecalis XC124
  4. cSEM, standard error of the means
  5. dD, delayed filling; I, inoculation; T, inoculating time; *P < 0.05; **P < 0.01; NS, not significant
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