Fermentation quality and bacterial community of delayed filling stylo silage in response to inoculating lactic acid bacteria strains and inoculating time

Chemical and Biological Technologies in Agriculture

Table 5 General information of sequence and bacterial diversity of stylo and its silage^a

Samples	Treatments^b	Effective Reads	OTUs	Chao 1	Simpson	Shannon	Coverage
Fresh stylo		10585	210	239	0.48b	2.45b	1.00
Stylo inoculated at chopping and placed for 2 d	CK	9974	197	231	0.87a	4.38a	0.99
	SXC48	9578	175	220	0.88a	4.32a	0.99
	CCZZ1	9769	179	203	0.90a	4.54a	1.00
	XC124	9482	174	218	0.85a	4.04a	0.99
SEM^c		142.540	5.437	4.530	0.050	0.238	0.000
Significance^d		NS	NS	NS	**	**	NS
Quick filling	CK	10530	120	160	0.59c	2.52c	1.00
	SXC48	9941	148	204	0.59c	2.37c	0.99
	CCZZ1	10279	148	184	0.68abc	2.63c	1.00
	XC124	10468	121	172	0.62bc	2.41c	1.00
Delayed filling (2 d)	CK	9994	128	167	0.78a	3.31abc	1.00
Inoculating at chopping	SXC48	10517	142	195	0.80a	3.65a	0.99
	CCZZ1	9779	120	222	0.78a	3.18abc	0.99
	XC124	9793	91	160	0.72abc	2.71bc	1.00
Inoculating at filling	SXC48	10120	100	161	0.76ab	2.97abc	1.00
	CCZZ1	10080	123	153	0.84a	3.57ab	1.00
	XC124	9652	105	148	0.77ab	3.07abc	1.00
SEM^c		76.032	4.552	6.471	0.020	0.103	0.000
Significance^d		NS	NS	NS	**	*	NS

Different lowercase letters in the same column and same material indicate significant difference (P < 0.05)
^aOTUs, operational taxonomic units
^bCK, without inoculation; SXC48 and CCZZ1, inoculating Lb. plantarum SXC48 and CCZZ1; XC124, inoculating En. faecalis XC124
^cSEM, standard error of the means
^d*P < 0.05; **P < 0.01; NS, not significant