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Chemical and Biological Technologies in Agriculture

Table 1 Tolerance of LAB strains to acid, bile salt and simulated gastrointestinal conditions

From: Evaluation of probiotic and anti-inflammatory properties of bacteriocinogenic Pediococcus acidilactici HW01 and Leuconostoc citreum HW02 from malted barley

 

Strain

Lb. rhamnosus GG

P. acidilactici HW01

Leu. citreum HW02

Acid (pH 2.5)

   

 Viable cell number (Log10 CFU/mL)

   

  0 h

6.59 ± 0.05

6.14 ± 0.09

6.15 ± 0.03

  1 h

6.59 ± 0.02

6.20 ± 0.01

6.26 ± 0.03

  2 h

6.47 ± 0.01

6.14 ± 0.02

6.14 ± 0.03

  3 h

6.41 ± 0.02

6.13 ± 0.06

6.14 ± 0.05

 Survival rate (%)

97.17 ± 1.06a

99.80 ± 0.61b

99.85 ± 0.62b

Bile salt (0.3%)

   

 Viable cell number (Log10 CFU/mL)

   

  0 h

7.05 ± 0.06

7.01 ± 0.06

7.15 ± 0.01

  1 h

7.09 ± 0.06

7.13 ± 0.04

7.19 ± 0.01

  2 h

7.13 ± 0.06

7.50 ± 0.11

7.42 ± 0.05

  3 h

7.27 ± 0.08

7.54 ± 0.07

7.88 ± 0.06

 Survival rate (%)

104.50 ± 1.74a

106.39 ± 0.74a

110.86 ± 1.50b

Bile salt (1%)

   

 Viable cell number (Log10 CFU/mL)

   

  0 h

7.10 ± 0.05

7.01 ± 0.06

7.10 ± 0.04

  1 h

7.12 ± 0.08

6.84 ± 0.12

7.30 ± 0.07

  2 h

7.13 ± 0.13

7.50 ± 0.11

7.43 ± 0.10

  3 h

7.24 ± 0.06

7.54 ± 0.07

7.64 ± 0.07

 Survival rate (%)

102.03 ± 1.35a

106.40 ± 1.65b

107.63 ± 0.89b

Lysozyme (100 mg/L)

   

 Viable cell number (Log10 CFU/mL)

   

  C*

8.93 ± 0.11

9.12 ± 0.13

9.04 ± 0.04

  L*

9.09 ± 0.07

9.08 ± 0.17

8.97 ± 0.07

 Survival rate (%)

101.81 ± 1.90b

99.66 ± 1.62a

99.20 ± 1.07a

Simulated gastrointestinal condition

   

Simulated stomach fluid (pH 2.5)

   

 Viable cell number (Log10 CFU/mL)

   

  0 h

7.30 ± 0.07

7.50 ± 0.08

7.50 ± 0.05

  1 h

7.26 ± 0.01

7.42 ± 0.07

7.44 ± 0.08

  2 h

7.26 ± 0.14

7.38 ± 0.05

7.44 ± 0.14

  3 h

7.38 ± 0.02

7.50 ± 0.03

7.55 ± 0.02

 Survival rate (%)

101.04 ± 1.12a

100.05 ± 0.91a

100.60 ± 0.92a

Simulated small intestinal fluid

   

 Viable cell number (Log10 CFU/mL)

   

  0 h

6.58 ± 0.17

6.29 ± 0.10

6.06 ± 0.08

  1 h

6.48 ± 0.20

6.13 ± 0.12

6.16 ± 0.13

  2 h

6.22 ± 0.17

6.14 ± 0.12

6.16 ± 0.12

  3 h

6.21 ± 0.15

6.16 ± 0.11

6.26 ± 0.13

 Survival rate (%)

94.40 ± 2.40a

97.43 ± 2.98a

103.69 ± 2.26b

  1. All values are shown as mean ± S.D. (Standard deviation) (n = 3)
  2. Lowercase letters (series “a–c”) indicate significant (Duncan’s range test, p < 0.05) differences in the same row
  3. C* and L* indicate control (without lysozyme) and lysozyme treatment (100 mg/L) samples, respectively
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