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Table 4 Zinc concentration and accumulation in shoot and grains tissues of common beans as a function of plant growth-promoting bacteria and nano-Zn foliar doses in 2019 and 2020 cropping seasons

From: Nano-zinc and plant growth-promoting bacteria is a sustainable alternative for improving productivity and agronomic biofortification of common bean

Treatments

Shoot Zn concentration (mg kg−1)

Grain Zn concentration (g ha−1)

Shoot Zn uptake (g ha−1)

2019

2020

2019

2020

2019

2020

Inoculations (I)

Control (no-inoculation)

30.6 ± 6.7 d

39.9 ± 5.0 d

43.6 ± 5.1

51.7 ± 3.7

118 ± 34.5

156 ± 26.5 d

 R. tropici

32.5 ± 5.3 c

42.0 ± 3.9 c

44.9 ± 2.5

54.6 ± 2.3

130 ± 25.1

167 ± 21.6 c

 R. tropici + A. brasilense

36.6 ± 6.7 b

44.7 ± 4.9 b

47.6 ± 4.7

56.3 ± 3.8

149 ± 31.2

182 ± 25.8 b

 R. tropici + B. subtilis

42.3 ± 4.5 a

49.3 ± 3.6 a

51.9 ± 2.1

60.6 ± 3.2

183 ± 21.1

210 ± 19.6a

 R. tropici + P. fluorescens

38.3 ± 5.4 b

45.8 ± 5.2 b

48.0 ± 5.4

58.2 ± 3.6

159 ± 28.5

189 ± 28.7 b

 R. tropici + A. brasilense + B. Subtilis

34.3 6.8 c

42.1 ± 4.5 c

45.6 ± 4.5

55.1 ± 4.0

139 ± 34.6

171 ± 25.9 c

 R. tropici + A. brasilense + P. fluorescens

32.7 ± 5.2 c

40.8 ± 2.8 d

44.1 ± 2.3

54.6 ± 2.6

129 ± 25.2

164 ± 14.1 c

Nano-Zn folair spray (kg ha−1)

 0.0

30.7 c

41.0 c

44.2

54.3

123

163 c

1.5

40.3 a

46.9 a

49.5

57.7

167

195 a

 3.0

34.9 b

42.6 b

45.8

55.7

141

174 b

F values

 I

31*

31*

43.5**

44**

57**

39**

Nano-Zn

105*

62*

88.9**

38**

129**

71**

 I × nano-Zn

1.6ns

0.74ns

4.9**

2.3*

3*

0.9ns

CV (%)

7

4.7

3.3

2.6

7

6

  1. Means in the column followed by similar letters are statistically not different by Tukey test (p ≤ 0.05) for foliar nano-Zn dose and Scott Knott test for PGPBs (p ≤ 0.05). ** and *—significant at p < 0.01 and p < 0.05, respectively, while ns—non-significant by F test. CV = Coefficient of variance. (n = 4 replicates)