Table 2 Summary of protein concentration and ChE activity results from crude purification at different salt levels
Specification | PBS extracted | Ammonium sulfate salt precipitation level (%) | |||||
|---|---|---|---|---|---|---|---|
0–30 | 30–50 | 50–75 | 0–40 | 0–50 | 0–75 | ||
Volume (mL) | 10.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 | 2.00 |
Protein (mg/mL) | 9.55 | 14.11 | 13.17 | 5.73 | 17.95 | 21.47 | 21.31 |
Total protein (mg) | 95.50 | 28.22 | 26.34 | 11.46 | 35.90 | 42.94 | 42.62 |
Total activity (µM/min) | 102.95 | 41.05 | 38.05 | 1.83 | 55.40 | 55.50 | 54.64 |
Specific activity (µM/min/mg) | 1.08 | 1.45 | 1.44 | 0.16 | 1.54 | 1.29 | 1.28 |
Purification factor (fold) | 1.00 | 1.35 | 1.34 | 0.15 | 1.43 | 1.20 | 1.19 |
Yield (%) | 100.00 | 39.88 | 36.96 | 1.77 | 53.81 | 53.91 | 53.07 |