16S metabarcoding, total soil DNA content, and functional bacterial genes quantification to characterize soils under long-term organic and conventional farming systems

Chemical and Biological Technologies in Agriculture

Table 2 Sequences of the primers used in this project and targeted amplicon length

Primer	Sequence	References
16S F	GGGTTGCGCTCGTTGC	[49]
16S R	ATGGYTGTCGTCAGCTCGTG	[49]
Archaeal amoA F	STAATGGTCTGGCTTAGACG	[50]
Archaeal amoA R	GCGGCCATCCATCTGTATGT	[50]
Bacterial amoA F	GGGGTTTCTACTGGTGGT	[51]
Bacterial amoA R	CCCCTCKGSAAAGCCTTCTTC	[51]
nifH F	AAAGGYGGWATCGGYAARTCCACCAC	[52]
nifH R	TTGTTSGCSGCRTACATSGCCATCAT	[52]
nosZ F	CGYTGTTCMTCGACAGCCAG	[52]
nosZ R	CATGTGCAGNGCRTGGCAGAA	[52]
nirK F	ATYGGCGGVCAYGGCGA	[53]
nirK R	RGCCTCGATCAGRTTRTGGTT	[53]
gh48 8F	CGCCCCABGMSWWGTACCA	[54]
gh48 5R	GCYTCCCAIATRTCCATC	[54]
PKS I F	GGCAACGCCTACCACATGCANGGNYT	[55]
PKS I R	GGTCCGCGGGACGTARTCNARRTC	[55]
PKS II F	TSGCSTGCTTGGAYGCSATC	[56]
PKS II R	TGGAANCCGCCGAABCCTCT	[56]
NRPS I F	CGCGCGCATGTACTGGACNGGNGAYYT	[55]
NRPS I R	GGAGTGGCCGCCCARNYBRAARAA	[55]
NRPS II F	GCSTACSYSATSTACACSTCSGG	[57]
NRPS II R	SASGTCVCCSGTSCGGTAS	[57]

The NCBI accession number is provided for all the primers designed on the base of a reference sequence obtained from this work’s metabarcoding