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Chemical and Biological Technologies in Agriculture
Fig. 1 | Chemical and Biological Technologies in Agriculture

Fig. 1

From: Transcriptome and Micro-CT analysis unravels the cuticle modification in phosphine-resistant stored grain insect pest, Tribolium castaneum (Herbst)

Fig. 1

Cuticle layer structure analysis using Micro-CT. a Scheme of sample preparation. b Summary of Micro-CT scanning and reconstruction process. c Post-processing of 3D rendering structure data for comparing parameters between phosphine-susceptible and resistant strain. Reconstructed cross-sectional images were thresholded with a grayscale index (254–255) (binarization). Cutting processing was only performed for 371 slides from first abdominal sterna to the posterior direction and the parts corresponding to the legs were cut (cutting). Removing noise from the inside (removal of inside noise) and outside (removal of outside noise) of the cuticle layer was followed

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