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Chemical and Biological Technologies in Agriculture
Fig. 7 | Chemical and Biological Technologies in Agriculture

Fig. 7

From: Transcriptome and Micro-CT analysis unravels the cuticle modification in phosphine-resistant stored grain insect pest, Tribolium castaneum (Herbst)

Fig. 7

Comparisons of total chitin contents and quantitative reverse transcription polymerase chain reaction (qRT-PCR) in phosphine (PH3)-susceptible (S) and resistant (R) Tribolium castaneum. a The number of adults per equivalent mass (0.5 g) for Calcofluor staining and the ratio of total chitin mass per single adult T. castaneum. b Scheme of chitin biosynthesis pathway representing differences between PH3-S and PH3-R strain of T. castaneum. c Relative gene expression level involved in chitin biosynthesis pathway using qRT-PCR. All data for chitin contents and relative mRNA level presented as mean ± standard deviation and analyzed using a t-test. Asterisk represents the statistic mark (*p < 0.05; **p < 0.01)

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