Table 1 Analysis of physicochemical properties and subcellular localization of DFR gene in Apple
From: Molecular evolution and expression assessment of DFRs in apple
Name | ID | Chromosome location | Amino acids | Molecular weight/D | pI | Instability index | Hydrophilic | Full length/bp | cDNA/bp | Subcellular localization |
|---|---|---|---|---|---|---|---|---|---|---|
MdDFR01 | MD00G1128000 | 0 | 465 | 52,188.03 | 8.61 | 32.12 | − 0.178 | 10,524 | 1398 | Cytoplasm/Plasma membrane |
MdDFR02 | MD01G1077400 | 1 | 341 | 37,566.81 | 5.37 | 36.2 | − 0.046 | 2458 | 1026 | Chloroplast |
MdDFR03 | MD01G1077900 | 1 | 342 | 37,702.34 | 6.72 | 31.8 | − 0.116 | 3089 | 1029 | Nucleus |
MdDFR04 | MD01G1125200 | 1 | 342 | 38,387.13 | 7.73 | 32.83 | − 0.37 | 3320 | 1029 | Cytoplasm |
MdDFR05 | MD02G1134100 | 2 | 372 | 41,821.12 | 6.32 | 49.93 | − 0.45 | 1952 | 1119 | Cytoplasm |
MdDFR06 | MD02G1205000 | 2 | 329 | 36,336.67 | 5.8 | 33.8 | − 0.064 | 3377 | 990 | Chloroplast |
MdDFR07 | MD02G1302800 | 2 | 324 | 35,564.88 | 6.02 | 33.99 | − 0.048 | 2888 | 975 | Chloroplast |
MdDFR08 | MD02G1302900 | 2 | 322 | 34,972.3 | 6.32 | 30.42 | 0.08 | 4285 | 969 | Chloroplast |
MdDFR09 | MD02G1303400 | 2 | 320 | 34,953.16 | 5.52 | 27.65 | − 0.019 | 2315 | 963 | Chloroplast |
MdDFR10 | MD02G1303600 | 2 | 75 | 8344.64 | 9.31 | 24.47 | − 0.409 | 885 | 228 | Cytoplasm |
MdDFR11 | MD02G1303800 | 2 | 520 | 56,745.3 | 5.86 | 30.74 | − 0.022 | 5546 | 1563 | Cytoplasm |
MdDFR12 | MD02G1303900 | 2 | 320 | 34,689.02 | 6 | 22.93 | 0.09 | 1748 | 963 | Chloroplast |
MdDFR13 | MD02G1304200 | 2 | 315 | 34,572.08 | 6.54 | 30.3 | 0.073 | 3088 | 948 | Chloroplast |
MdDFR14 | MD02G1304400 | 2 | 326 | 35,846.39 | 6.26 | 32.13 | 0.028 | 3818 | 981 | Cytoplasm |
MdDFR15 | MD02G1304900 | 2 | 326 | 35,504.95 | 6.25 | 28.63 | 0 | 3735 | 981 | Chloroplast |
MdDFR16 | MD02G1305300 | 2 | 320 | 34,828.07 | 5.68 | 33.35 | 0.016 | 2152 | 963 | Chloroplast |
MdDFR17 | MD03G1096800 | 3 | 346 | 39,263.97 | 6.53 | 29.46 | − 0.438 | 4203 | 1041 | Chloroplast |
MdDFR18 | MD03G1131500 | 3 | 363 | 40,762.7 | 5.49 | 48.89 | − 0.233 | 2479 | 1092 | Cytoplasm |
MdDFR19 | MD03G1131900 | 3 | 193 | 21,672.97 | 6.82 | 40.99 | − 0.018 | 1199 | 582 | Cytoplasm |
MdDFR20 | MD03G1172800 | 3 | 325 | 35,447.67 | 6.02 | 28.25 | − 0.046 | 6365 | 978 | Cytoplasm |
MdDFR21 | MD03G1214100 | 3 | 364 | 39,373.58 | 6.78 | 37.93 | 0.235 | 6025 | 1095 | Chloroplast |
MdDFR22 | MD03G1250400 | 3 | 673 | 75,908.82 | 6.62 | 34.16 | − 0.345 | 3075 | 2022 | Chloroplast |
MdDFR23 | MD03G1288200 | 3 | 439 | 49,147.28 | 8.72 | 37.33 | − 0.303 | 4475 | 1320 | Cytoplasm |
MdDFR24 | MD04G1006100 | 4 | 315 | 34,687.1 | 5.82 | 34.54 | 0.06 | 2882 | 948 | Chloroplast |
MdDFR25 | MD04G1064600 | 4 | 350 | 38,830.36 | 6.56 | 24.02 | − 0.283 | 3455 | 1053 | Cytoplasm |
MdDFR26 | MD04G1118200 | 4 | 428 | 47,962.79 | 8.91 | 36.4 | − 0.366 | 3360 | 1287 | Mitochondria |
MdDFR27 | MD04G1224100 | 4 | 459 | 51,977.55 | 7.59 | 39.65 | − 0.298 | 3314 | 1380 | Cytoplasm |
MdDFR28 | MD04G1224200 | 4 | 376 | 42,590.4 | 5.99 | 39.74 | − 0.455 | 3467 | 1131 | Cytoskeleton |
MdDFR29 | MD05G1099800 | 5 | 324 | 35,547.83 | 6.76 | 18.26 | − 0.052 | 1461 | 975 | Cytoplasm |
MdDFR30 | MD05G1145800 | 5 | 142 | 15,915.48 | 5.43 | 38.67 | 0.164 | 617 | 429 | Extracellular matrix |
MdDFR31 | MD05G1148900 | 5 | 327 | 36,450.14 | 6.71 | 34.49 | − 0.074 | 1747 | 984 | Chloroplast |
MdDFR32 | MD05G1230600 | 5 | 465 | 51,041.33 | 9.58 | 36.9 | − 0.254 | 1398 | 1398 | Cytoplasm/E,R |
MdDFR33 | MD05G1263800 | 5 | 418 | 45,991.49 | 8.42 | 39.05 | − 0.24 | 5579 | 1257 | Mitochondria |
MdDFR34 | MD05G1335600 | 5 | 339 | 36,873.53 | 5.52 | 35.57 | 0.008 | 2865 | 1020 | Chloroplast |
MdDFR35 | MD06G1003200 | 6 | 287 | 32,201.16 | 5.59 | 33.48 | − 0.139 | 2409 | 864 | Chloroplast |
MdDFR36 | MD06G1003300 | 6 | 315 | 35,070.25 | 6.95 | 37.45 | − 0.168 | 3385 | 948 | E,R |
MdDFR37 | MD06G1003500 | 6 | 339 | 37,957.84 | 5.89 | 34.05 | − 0.086 | 3376 | 824 | Chloroplast |
MdDFR38 | MD06G1003700 | 6 | 315 | 35,290.7 | 7.54 | 36.28 | − 0.181 | 2515 | 948 | Peroxisome |
MdDFR39 | MD06G1003800 | 6 | 310 | 34,978.2 | 6.67 | 39.19 | − 0.286 | 2033 | 933 | Cytoplasm |
MdDFR40 | MD06G1075100 | 6 | 84 | 9752.16 | 9.26 | 43.83 | − 0.502 | 353 | 255 | Extracellular matrix |
MdDFR41 | MD06G1103900 | 6 | 344 | 37,444.75 | 5.17 | 38.9 | − 0.01 | 2404 | 1035 | Chloroplast |
MdDFR42 | MD06G1108600 | 6 | 384 | 41,987.91 | 6.66 | 33.72 | − 0.123 | 3709 | 1155 | Chloroplast |
MdDFR43 | MD07G1020400 | 7 | 301 | 32,386.19 | 6.17 | 30.71 | 0.033 | 1771 | 906 | Chloroplast |
MdDFR44 | MD07G1020900 | 7 | 321 | 34,990.43 | 6.33 | 30.32 | 0.055 | 6884 | 966 | Cytoplasm |
MdDFR45 | MD07G1021200 | 7 | 321 | 35,146.63 | 5.75 | 30.64 | 0.039 | 2461 | 966 | Chloroplast |
MdDFR46 | MD07G1021600 | 7 | 424 | 47,000.12 | 6.84 | 26.56 | − 0.071 | 5868 | 1275 | Chloroplast |
MdDFR47 | MD07G1119800 | 7 | 438 | 48,556.83 | 9.96 | 41.94 | − 0.239 | 1491 | 1317 | E,R |
MdDFR48 | MD07G1120100 | 7 | 438 | 48,401.62 | 9.88 | 42.55 | − 0.216 | 1317 | 1317 | E,R |
MdDFR49 | MD07G1121700 | 7 | 265 | 29,593.13 | 6.5 | 37.64 | 0.029 | 1737 | 798 | Chloroplast |
MdDFR50 | MD07G1194900 | 7 | 342 | 38,432.17 | 7.73 | 33.07 | − 0.363 | 3056 | 1029 | Cytoplasm |
MdDFR51 | MD07G1256800 | 7 | 407 | 45,249.17 | 9.89 | 38.41 | − 0.105 | 1224 | 1224 | Mitochondria |
MdDFR52 | MD08G1024600 | 8 | 343 | 38,584.95 | 9.06 | 46.41 | − 0.491 | 2696 | 1032 | Chloroplast |
MdDFR53 | MD08G1028600 | 8 | 347 | 38,938.78 | 6.02 | 31.36 | − 0.19 | 2740 | 1044 | Chloroplast |
MdDFR54 | MD08G1063500 | 8 | 130 | 14,689.74 | 5.49 | 33.33 | 0.014 | 393 | 393 | Cytoplasm/Cytoskeleton |
MdDFR55 | MD08G1103100 | 8 | 340 | 37,107.64 | 8.87 | 25.57 | 0.008 | 1611 | 1023 | Chloroplast |
MdDFR56 | MD08G1231300 | 8 | 267 | 29,735.22 | 6.54 | 33.32 | − 0.086 | 1230 | 804 | Cytoplasm |
MdDFR57 | MD08G1231400 | 8 | 331 | 36,275.57 | 6.15 | 28.92 | 0.001 | 7363 | 996 | Chloroplast |
MdDFR58 | MD08G1231500 | 8 | 331 | 36,464.83 | 5.93 | 33.05 | 0.02 | 3253 | 996 | Chloroplast |
MdDFR59 | MD08G1250300 | 8 | 333 | 36,345.35 | 5.79 | 36.65 | − 0.097 | 2531 | 1002 | Cytoplasm |
MdDFR60 | MD09G1137100 | 9 | 492 | 54,643.35 | 8.26 | 41.98 | − 0.131 | 3450 | 1479 | Chloroplast |
MdDFR61 | MD09G1154200 | 9 | 349 | 39,647.33 | 8.77 | 23.03 | − 0.321 | 1050 | 1050 | Chloroplast/Peroxisome |
MdDFR62 | MD09G1154300 | 9 | 755 | 85,437.77 | 8.89 | 33.75 | − 0.368 | 3160 | 2268 | Cytoskeleton |
MdDFR63 | MD09G1154700 | 9 | 439 | 49,246.31 | 7.6 | 35.14 | − 0.313 | 2577 | 1320 | Chloroplast |
MdDFR64 | MD09G1224200 | 9 | 339 | 37,321.48 | 5.77 | 34.59 | − 0.277 | 6111 | 1020 | Chloroplast |
MdDFR65 | MD10G1105500 | 10 | 324 | 35,560.83 | 6.8 | 19.26 | − 0.066 | 1427 | 975 | Chloroplast |
MdDFR66 | MD10G1145200 | 10 | 388 | 42,974.74 | 6.82 | 46.02 | − 0.058 | 4041 | 1167 | Nucleus |
MdDFR67 | MD10G1145300 | 10 | 357 | 39,608.62 | 6.36 | 39.52 | − 0.095 | 1804 | 1074 | Extracellular matrix |
MdDFR68 | MD10G1241500 | 10 | 418 | 46,024.61 | 8.61 | 35.93 | − 0.236 | 5481 | 1257 | Mitochondria |
MdDFR69 | MD10G1311100 | 10 | 339 | 36,781.43 | 5.29 | 34.43 | 0.027 | 4079 | 1020 | Chloroplast |
MdDFR70 | MD11G1063800 | 11 | 1204 | 136,293.11 | 6.6 | 36.11 | − 0.196 | 5680 | 3615 | Nucleus |
MdDFR71 | MD11G1064000 | 11 | 324 | 35,481.08 | 6.16 | 26.84 | − 0.005 | 3081 | 975 | Chloroplast |
MdDFR72 | MD11G1064600 | 11 | 324 | 35,447.98 | 5.88 | 25.43 | 0.004 | 2792 | 975 | Cytoplasm |
MdDFR73 | MD11G1064700 | 11 | 324 | 35,522.98 | 5.58 | 23.38 | − 0.003 | 2775 | 975 | Cytoplasm |
MdDFR74 | MD11G1143300 | 11 | 346 | 39,263.04 | 7.74 | 29.68 | − 0.449 | 4429 | 1041 | Chloroplast |
MdDFR75 | MD11G1154500 | 11 | 389 | 43,741.02 | 5.71 | 48.47 | − 0.295 | 3724 | 1170 | Cytoplasm |
MdDFR76 | MD11G1190800 | 11 | 325 | 35,552.86 | 6.61 | 26.41 | − 0.056 | 5545 | 978 | Chloroplast |
MdDFR77 | MD11G1215900 | 11 | 100 | 11,167.15 | 5.53 | 41.48 | 0.408 | 525 | 303 | Extracellular matrix |
MdDFR78 | MD11G1229100 | 11 | 260 | 28,157.07 | 5.02 | 41.73 | 0.147 | 2656 | 783 | Cytoplasm |
MdDFR79 | MD11G1270500 | 11 | 461 | 52,631.28 | 7.14 | 37.19 | − 0.482 | 1802 | 1386 | Cytoskeleton |
MdDFR80 | MD11G1285100 | 11 | 403 | 43,716.68 | 7.16 | 44.6 | − 0.172 | 5323 | 1212 | Chloroplast |
MdDFR81 | MD11G1309900 | 11 | 234 | 26,339.04 | 6.66 | 28.68 | − 0.492 | 3635 | 705 | Chloroplast |
MdDFR82 | MD12G1133600 | 12 | 445 | 49,829.97 | 8.76 | 35.09 | − 0.275 | 2843 | 1338 | Cytoplasm |
MdDFR83 | MD12G1240900 | 12 | 381 | 43,184.13 | 5.99 | 39.41 | − 0.149 | 3415 | 1146 | Cytoskeleton |
MdDFR84 | MD13G1042500 | 13 | 319 | 35,187.18 | 6.25 | 38.69 | − 0.134 | 2041 | 960 | Cytoplasm/Chloroplast |
MdDFR85 | MD13G1274600 | 13 | 432 | 47,898.48 | 9.63 | 38.01 | − 0.263 | 1299 | 1299 | Cytoplasm |
MdDFR86 | MD14G1129500 | 14 | 347 | 38,036.26 | 6.46 | 28.81 | − 0.175 | 3965 | 1044 | Cytoplasm |
MdDFR87 | MD15G1024100 | 15 | 348 | 39,029.79 | 5.68 | 36.2 | − 0.218 | 2708 | 1047 | Chloroplast |
MdDFR88 | MD15G1058400 | 15 | 483 | 52,393.5 | 8.23 | 35.08 | − 0.252 | 2243 | 1452 | Chloroplast |
MdDFR89 | MD15G1106300 | 15 | 660 | 74,793.14 | 6.55 | 28.31 | − 0.385 | 2161 | 1983 | Cytoplasm |
MdDFR90 | MD15G1247100 | 15 | 372 | 41,806.11 | 6.36 | 48.59 | − 0.457 | 1139 | 1119 | Cytoplasm |
MdDFR91 | MD16G1167000 | 16 | 437 | 48,713.5 | 8.31 | 34.9 | − 0.315 | 3282 | 1314 | Chloroplast |
MdDFR92 | MD16G1216800 | 16 | 157 | 17,742.56 | 6.17 | 27.73 | 0.145 | 1099 | 474 | Cytoplasm/Chloroplast |
MdDFR93 | MD16G1260200 | 16 | 432 | 47,877.41 | 9.54 | 39.65 | − 0.244 | 1299 | 1299 | Cytoplasm |
MdDFR94 | MD17G1127400 | 17 | 364 | 40,246.83 | 8.78 | 35.54 | − 0.292 | 8743 | 1095 | Cytoplasm |
MdDFR95 | MD17G1140800 | 17 | 745 | 84,110.34 | 7.27 | 34.43 | − 0.265 | 3721 | 2238 | Chloroplast/Vacuole |
MdDFR96 | MD17G1222400 | 17 | 339 | 37,176.33 | 6.02 | 32.71 | − 0.24 | 6210 | 1020 | Chloroplast |