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Chemical and Biological Technologies in Agriculture
Fig. 2 | Chemical and Biological Technologies in Agriculture

Fig. 2

From: JUNB affects hair follicle development and regeneration by promoting the proliferation of dermal papilla cells in goat

Fig. 2

Effect of JUNB on DP cells viability, proliferation, and cell cycle. A Flow cytometry analysis of DP cells cycle after pAd-JUNB and pAd-NC infection for 48 h. Statistical analysis of the proportion of cells in each cell cycle. B EdU staining analysis of DP cells proliferation following 48 h of pAd-JUNB and pAd-NC infection treatments. Statistical analysis of the number of EdU-positive cells. C MTT analysis of DP cells viability after pAd-JUNB and pAd-NC infection for 48 h. D CCK-8 analysis of DP cells viability after pAd-JUNB and pAd-NC infection for 48 h. E RT-qPCR result of cell proliferation marker gene PCNA and MKi67 after pAd-JUNB and pAd-NC infection for 48 h. F Immunofluorescence staining of PCNA and Ki67 after pAd-JUNB and pAd-NC infection for 48 h. The data are expressed as the mean ± SE (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. Scale bars, 100 μm

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