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Chemical and Biological Technologies in Agriculture
Fig. 3 | Chemical and Biological Technologies in Agriculture

Fig. 3

From: JUNB affects hair follicle development and regeneration by promoting the proliferation of dermal papilla cells in goat

Fig. 3

The mechanism verification of JUNB regulating TCF3. A The DNA-binding motif of JUNB from JASPAR database. B The transcription binding sites of JUNB on the TCF3 promoter region. C Dual-luciferase assay to detect the optimal promoter activity of TCF3 in HEK293T cells. D The mutation of JUNB transcriptional binding sites on the TCF3 promoter region, and dual-luciferase assay to detect the interaction between JUNB and TCF3 in HEK293T cells. Binding sites are marked in red and mutated binding sites are marked in blue. E RT-qPCR analysis of TCF3 expression after pAd-JUNB and pAd-NC infection for 48 h in DP cells. F Immunofluorescence staining of TCF3 after pAd-JUNB and pAd-NC infection for 48 h in DP cells. The mean fluorescence intensity was analyzed by Image J. IntDen is the total fluorescence intensity of the region, and area is the extent of the region. The data are expressed as the mean ± SE (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001. Scale bars, 100 μm

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