Table 1 Real-time quantitative PCR primer information
Gene name | Accession no (Gene bank) | Primer sequence (5ʹ-3ʹ) | Product size (bp) | Tm (°C) |
|---|---|---|---|---|
JUNB | XM_005682285.3 | F: CAAGGGACACGCCTTCTGAG | 166 | 60 |
| Â | Â | R: CAGGAGTCCAGTGTGGTTTG | Â | Â |
PCNA | XM_005688167.3 | F: AAGAGGAGGAAGCTGTTACC | 198 | 60 |
| Â | Â | R: CGATCTTGGGAGCCAAATAG | Â | Â |
MKi67 | XM_005698601.3 | F: GGACATTCAGACCCTAAGTG | 180 | 60 |
| Â | Â | R: GTCTCGACGACTTCTGGTTC | Â | Â |
TCF3 | XM_018050411.1 | F: CCGTGTCGGTCATCCTGAAC | 112 | 60 |
| Â | Â | R: TCTCCAACCACGCCTGATAC | Â | Â |
CTNNB1 | XM_018066894.1 | F:GAGGACAAGCCACAGGATTAT | 101Â | 58Â |
| Â | Â | R: CCAAGATCAGCGGTCTCATT | Â | Â |
Cyclin D1 | XM_018043271.1 | F: GCAGTCTTAGGCATCCTGTAT | 131 | 58 |
| Â | Â | R: CCTAGCCGAGAGGTTACATTA | Â | Â |
C-myc | XM_018058563.1 | F: CAGAGGAGAAACGAGCTGAAA | 130 | 62 |
| Â | Â | R: CTTGGACCGACAGGATGTATG | Â | Â |
β-actin | NM_001314342.1 | F: TGAACCCCAAAGCCAACC | 107 | 58 |
| Â | Â | R: AGAGGCGTACAGGGACAGCA | Â | Â |