Skip to main content

Table 1 Specific forward and reverse primer sequences used in semi-quantitative RT-PCR

From: Humic substances stimulate maize nitrogen assimilation and amino acid metabolism at physiological and molecular level

Gene Primer forward (5′-3′) Primer reverse (5′-3′) Accession number
NR (nitrate reductase) 5′-TTCATGAACACTACCGACGTCG-3′ 5′-GAGCCTGTACGGATACTCGGC-3′ M27821
AS (asparagine synthetase) 5′-CATCATTGAGCTCTCGCGCAGGTTAC-3′ 5′-GGGGGAAATGTTATGAAGCGTTCACAA-3′ X82849
CS (citrate synthetase) 5′-GTTTGGTCATGGAGTTCTGCGTAA-3′ 5′-GGAGGTACAACTTCATACAACTTGGACAC-3′ W49861
IDH (isocitrate dehydrogenase) 5′-AAACTCGAGGCTGCTTGCGTTGAGA-3′ 5′-ATAATTAGCTTGCATCGAAACTGCGG-3′ W21690
MDH (malate dehydrogenase) 5′-GCCAGATTTCTGAGAGACTTAATGTCCA-3′ 5′-TCGAGGCATGAGTAAGCAAGCGTCTT-3′ T27564
18S 5′-CCATCCCTCCGTAGTTAGCTTCT-3′ 5′-CCTGTCGGCCAAGGCTATATAC-3′ U42796.1