Skip to main content

Anticandidal effects and chemical compositions of volatile oils extracted from Origanum syriacum, Clinopodium serpyllifolium subsp. fruticosum and Thymbra capitata from Palestine



Over the past decade, researchers have been exploring the potential therapeutic benefits of volatile oils (VOs) in addressing various disorders, particularly those associated with an increase in fungal infections. This study aimed to analyze the chemical compositions of three different thyme species growing in Palestine using gas chromatography–mass spectroscopy (GC–MS) and explore their antifungal characteristics. The thyme species investigated in this research encompass Origanum syriacum L., Clinopodium serpyllifolium subsp. fruticosum (L.) Bräuchler, and Thymbra capitata (L.) Cav.


The VOs of the investigated plants were extracted by hydrodistillation technique equipped with Cleavenger apparatus and characterized by utilizing GC–MS equipment. Moreover, the extracted VOs were evaluated for their antifungal activity using the broth microdilution assay against several clinically isolated Candida species and one ATCC strain.


The GC–MS characterization results of O. syriacum VO revealed the presence of 22 components and the abundant molecules were thymol (37.36%), carvacrol (27.71%), γ-terpinene (17.47%), and p-cymene (7.80%), while 19 compounds were characterized in the C. serpyllifolium VO and the major components were p-cymene (37.58%), carvacrol (22.93%), and γ-terpinene (21.91%). In addition, 23 compounds were identified in T. capitata VO and the main components were carvone (59.45%), pulegone (21.59%), menthone (4.24%), and isomenthone (3.71%). According to the antifungal assay results, VO extracted from O. syriacum has the highest activity among all the screened VOs.


All the VOs screened in this study exhibit promising antifungal activities for various potential medical applications. Consequently, we strongly advocate for further biological investigations of these oils in the near future.

Graphical Abstract


In recent years, there has been a noticeable increase in interest in investigating the medicinal potential of plants, with the majority of these studies focusing on historically utilized plants for food and medicine [1,2,3]. These natural remedies offer several advantages, including fewer side effects, cost-effectiveness, and non-toxic properties than synthetic drugs [4]. A contemporary treatment method has emerged that combines herbal supplements with traditional chemotherapeutic drugs to increase efficacy while minimizing side effects [5]. However, herbal medicine is not subjected to the same regulatory processes as conventional medicine [6,7,8].

This investigation seeks to evaluate the therapeutic potential of chemicals found in the VOs of three Palestinian thyme species: Origanum syriacum L. syriacum is a perennial aromatic herb that is native to western Asia, southern Europe, and the eastern Mediterranean [9]. It can be found growing in the open or under cultivation. The leaves, which are commonly used as Arab seasonings, have been incorporated into baked goods, teas, fragrances, and flavors, as well as traditional, complementary, and alternative medicine [10]. Clinopodium serpyllifolium subsp. fruticosum (L.) Bräuchler is a profusely aromatic perennial herbaceous plant in Palestine's flora. This plant's leaves and flowers are loaded with potent phytochemicals that have been used to treat a variety of medical conditions for centuries [11]. Its medicinal characteristics, such as its antimicrobial potential, have been the subject of several investigations [12].

Thymbra capitata (L.) Cav. is an aromatic medicinal and culinary herb that grows in a variety of Mediterranean regions and possesses significant therapeutic properties that are primarily attributable to its VO constituents [13]. The VO of T. capitata is highly valued and economically significant due to its unique biological properties. Previous investigations revealed many potential effects, one of which is antimicrobial activity [14].

Candida is a yeast genus that is one of the most common causes of fungal infections worldwide [15]. Candida species are found as endosymbionts in a variety of human body locations, including the urogenital tract, the gastrointestinal tract, and the skin [16]. While women are more susceptible to vaginal yeast infections, men can also be affected. Certain factors, such as long-term antibiotic use, put both men and women at risk. Fungal infections are more common in diabetics and people who are immunocompromised [17, 18]. A number of Candida species are well known in medicine for causing diverse illnesses, with C. albicans, C. parapsilosis, C. tropicalis, and C. glabrata causing the majority of opportunistic infections. These are either superficial or systemic infections of adults and neonates involving the bloodstream, urinary tract, vagina, esophagus, and oral cavity [19,20,21,22].

Numerous studies have explored the antifungal properties of VOs, focusing on their potential to hinder the growth of fungal pathogens [23,24,25]. While significant headway has been made in understanding these effects, a noticeable research gap pertains to the distinct chemotypes of VOs from different plant sources and their effectiveness against fungal infections. Moreover, a fresh approach to combat Candida infections has emerged: targeting essential functions crucial for the pathogen's virulence [26].

In this study, we looked at the chemical compositions and antifungal activities of VOs obtained from three flourish thyme species (Lamiaceae family): O. syriacum (local name: زعترعادي), C. serpyllifolium subsp. fruticosum (local name: زعتر فارسي) and T. capitata. (local name: زعتربلاط).

Material and methods

Plant samples collection, preparation, and extraction of VOs

During the flowering time, O. syriacum, C. serpyllifolium, and T. capitata leaves were gathered from the northern regions of Palestine in May 2021. The plants' samples were recognized by the pharmacognosist, Dr. Nidal Jaradat. A voucher specimen was deposited at the An-Najah National University in the Herbal Products Laboratory and deposed with the codes Pharm-PCT-A1729, Pharm-PCT-1575, and Pharm-PCT-690, respectively) at the same laboratory.

The herbs fresh leaves were dried in the shade at room temperature and relative humidity (25 ± 2  C, 55 ± 3 RH), respectively. The dried parts were pulverized to a fine powder of 60 μm in diameter and stored in airtight containers with adequate labeling for future use. All chemicals and reagents were of analytical grade and were supplied from Sigma-Aldrich (Germany). The VOs were extracted by the hydrodistillation technique [27]. Briefly, 100 mg of the dried leaves powder was suspended with 1 L of distilled water, and the VOs were extracted utilizing hydrodistillation with Clevenger apparatus operating at atmospheric pressure for 110 min at 100 °C. The obtained oils were chemically dried utilizing magnesium sulfate and stored at 2 °C until further use.

Gas chromatography/mass spectrometry (GC–MS)

Perkin Elmer Clarus 500 Gas Chromatograph apparatus was utilized for the characterizations of the O. syriacum, C. serpyllifolium, and T. capitata VOs components. This apparatus was connected to the Perkin Elmer Clarus 560 mass spectrometer. The separation was achieved by Perkin Elmer Elite-5-MS fused silica capillary column (film thickness 0.25 µm, 30 m × 0.25 mm). The components were recognized by associating the mass spectra of the components with authentic samples and/or the data from NIST, by interpreting the mass spectral fragmentation pattern of the molecules, and by relating retention indices (RIs) computed relative to a reference mixture of n-alkanes (C6–C30) [28].

Antifungal activity

Broth microdilution assay was used in the current study to estimate the antifungal activity of O. syriacum, C. serpyllifolium, and T. capitata VOs against clinically isolated Candida isolates and reference ATCC (90028) Candida albicans.

The VOs were dissolved in DMSO to a concentration of 100 µg/mL. The solution was serially micro-diluted (twofold) 10 times in sterile Brain Heart Infusion (BHI) broth. The dilution process was performed under aseptic conditions in 96-well plates. Wells number eleven contained plant-free BHI broth, which was used as a positive control for microbial growth. Wells number 12 contained plant-free and microbial-free BHI broth, which was used as a negative control for microbial growth. Wells 1–11 were inoculated aseptically with the test microbes. All the inoculated plates were incubated at 35 °C for approximately 48 h. The lowest VOs concentration with no visible microbial growth was considered to be the minimal inhibitory concentration (MIC). Fluconazole was used as a positive control for the antifungal activity [29, 30]. The VOs' antimicrobial activity was assessed in triplicate.

Results and discussion

Phytochemical GC–MS analysis

GC–MS analysis of O. syriacum VO revealed the presence of 22 components, accounting for 100% of the total VO. Approximately 97.05% are oxygenated monoterpenes and monoterpene hydrocarbons. Among the abundant identified compounds, were thymol (37.36%), carvacrol (27.71%), γ-terpinene (17.47%), and p-cymene (7.80%) (Table 1). The VO of C. serpyllifolium was also examined, and the GC–MS analysis revealed the presence of 19 distinct compounds. Monoterpene hydrocarbons (69.70%) and oxygenated monoterpenes (24.46%) constituted the largest groupings in VO. The most prevalent components were p-cymene (37.58%), carvacrol (22.93%), and γ-terpinene (21.91%) as shown in Table 2. Regarding T. capitata's VO, it consisted of 23 distinct compounds, accounting for 99.82% of the total VO. Oxygenated monoterpenes (94.53%) were the predominant phytochemical class of the oil, which was composed of carvone (59.45%), pulegone (21.59%), methone (4.24%) and isomenthone (3.71%) as the principal constituents (Table 3).

Table 1 Chemical composition of Origanum syriacum volatile oil by GC/MS
Table 2 Chemical composition of Clinopodium serpyllifolium subsp. fruticosum volatile oil by GC/MS
Table 3 Chemical composition of Thymbra capitata volatile oil by GC/MS

Comparing our findings to those of Al Hafi et al.2016 [31], the main components of the VO of O. syriacum from Lebanon differ from Palestinian O. syriacum VO. In detail, the Lebanese O. syriacum VO mainly contains carvacrol (79%), whereas the carvacrol consists 27.71% from the Palestinian O. syriacum VO while thymol is the major component with 37.36%. Interestingly, thymol’s anticandidal effect has been reported in other studies [24, 32, 33]. This variation in the chemical composition of the compositions of VOs from different populations of O. syriacum highlights the influence of environmental factors such as location and climate on the plant's secondary metabolites. Different dominating components, like carvacrol and thymol, may be responsible for variations in the VO’s potential bioactivity and medicinal effects. The monoterpenes category constitutes the predominant compounds in VOs known for their anticandidal properties. Notably, these include p-cymene (found in 40 plants), linalool (in 35 plants), γ-terpinene (in 33 plants), carvacrol (in 31 plants), 1-8-cineole (in 30 plants), α-pinene (in 28 plants), and thymol (in 27 plants). Additionally, the sesquiterpene β-caryophyllene is present in 15 out of 100 plants, which could contribute to the expected antifungal activity based on the resulting chemical composition of various plants [26].

Regarding the Palestinian T. capitata, in comparison with those collected from Sicily and Spain reported by Verdeguer et al. [14], it was found that the carvacrol is the major component of T. capitata VO from Sicili (77.02%) and from Spain (77.13%), whereas carvone (59.45%) was identified as major components of the T. capitata VO growing in Palestine. These findings highlight the consistent presence of carvacrol in T. capitata VO and emphasize its significance in anticandidal effect that is also reported by Manohar et al. [25].

Antifungal activity

Due to their toxic nature and increasing resistance to current antifungal drugs, the treatment of Candida infections has become challenging. As a result, there is a pressing need for new antifungal agents and alternative approaches, particularly those derived from natural sources [34].

In recent years, the prevalence of fungal infections has risen, particularly among immunocompromised individuals such as those with HIV infection, those undergoing chemotherapy, and organ or bone marrow transplant recipients. These individuals are susceptible to various forms of fungal infections. Candida infections are extremely common in these individuals, resulting in oral, vaginal, and/or systemic candidiasis [35]. Furthermore, Abdalrazeq et al. [36] conducted a study on the Thymbra plant from Palestine, comparing it with the Turkish Thymbra previously analyzed by Baydar et al. [37], the VOs derived from Turkish Thymbra exhibited microbial growth inhibition at concentrations below 1/100 (v/v), whereas the Palestinian Thymbra demonstrated antimicrobial effects at concentrations below 1/800. This observation suggests that the Palestinian Thymbra extract exerts stronger antimicrobial activity than its Turkish counterpart, likely attributed to regional differences between the two plants.

In the present study, the antifungal activity of the screened VOs against Candida species was evaluated using the microbroth dilution assay. The results showed that all the tested VOs exhibited antifungal effects. Notably, the VOs extracted from O. syriacum and C. serpyllifolium have the highest anticandidal activity against the C. albicans ATCC strain. While the T. capitata MIC value was very close to fluconazole. Interestingly, the VO extracted from O. syriacum revealed the highest potential against the clinical strains of C. parapsilosis, C. tropicalis, and C. albicans which is also of higher potential than fluconazole. Moreover, C. serpyllifolium VO showed strong inhibitory effects almost on the growth of all clinical strains. Finally, all tested VOs displayed strong antifungal activity compared to fluconazole, which was used as a positive control for the antifungal assay Table 4.

Table 4 Antifungal microbroth dilution assay MIC (µg/mL) values of T. capitata, C. serpyllifolium and O. syriacum VOs

The observed potent anticandidal effects of the VOs extracted from O. syriacum, C. serpyllifolium, and T. capitata can be attributed to the unique chemical compositions of these plant VOs. In O. syriacum VO, the dominant presence of thymol, carvacrol, γ-terpinene, and p-cymene plays a pivotal role. Thymol and carvacrol, renowned for their antimicrobial properties, likely contribute significantly to the robust antifungal activity. These phenolic compounds are known to disrupt cell membranes and vital cellular processes, leading to Candida species' growth inhibition [38]. Similarly, C. serpyllifolium VO's effectiveness can be linked to its abundant p-cymene, carvacrol, and γ-terpinene content, all acknowledged for their anticandidal potential [39, 40]. These compounds may collaborate synergistically to exert strong inhibitory effects. In T. capitata, the high proportion of oxygenated monoterpenes, particularly carvone and pulegone, underpins the VO's potency. Carvone exhibits antifungal properties through membrane disruption, while pulegone may contribute to this effect and potentially target specific pathways in Candida species [41]. The minor compounds in these VOs might also contribute synergistically or independently to the observed activity. Overall, the distinctive chemical profiles of these plant VOs, rich in well-known antimicrobial components, likely underlie their potent anticandidal effects. Further mechanistic studies could shed light on the exact interactions and pathways that contribute to the observed activities.

In summary, the VO extracted from O. syriacum has the highest anticandidal activity among all the screened VOs. However, it is worth noting that previous studies (Table 5) have also reported promising anticandidal activity of T. capitata, C. serpyllifolium, and O. syriacum VOs against clinical strains of C. glabrata, C. albicans, C. tropicalis, and C. parapsilosis.

Table 5 Previous investigations examined the antifungal activity of T. capitata, C. serpyllifolium, and O. syriacum VOs against C. glabrata, C. albicans, C. tropicalis, and C. parapsilosis using a microbroth dilution test (MIC values)

Considering the intricate chemical composition of plants’ oils and the tendency for biological effects to stem from synergistic interactions among their diverse components, pinpointing the primary active compounds is challenging. The assessment of MIC values on reference strains of C. albicans suggests that certain monoterpenes and their derivatives play a crucial role in plants’ oils exhibiting potent antifungal properties. Notably, compounds such as terpinyl acetate, α-terpineol, β-linalool, and γ-terpinene demonstrate robust anti-Candida activity when they constitute major constituents within the oils as in the case of Salvia mirzayaniiPlectranthus caninus Roth, and Thymus willdenowii Boiss [44, 45]. Conversely, the compounds comprising a significant portion of the plants’ oil may not always be directly accountable for their activity. This further complicates the challenge of establishing a direct correlation between the plants’ chemical composition and its biological effectiveness.

Extensive research has been dedicated to exploring the antifungal potential of individual acyclic and cyclic monoterpenes [46, 47]. Among the tested compounds, α-terpineol, terpinen-4-ol, 1,8-cineol, and β-linalool have been documented to exhibit particularly swift fungicidal activity. In contrast, γ-terpinene, α-terpinene, terpinolene, and p-cymene displayed a somewhat slower yet still substantial antifungal effect. This dichotomy implies that the presence of the alcohol functional group holds greater significance than the specific cyclic or acyclic structure, concerning the rapid inhibitory impact on fungal growth. This phenomenon is attributed to the superior water solubility of alcohols within aqueous environments and microbial membranes [48, 49]. The recognition of plant species exhibiting notable anti-Candida activity could serve as a foundational step for a taxonomic strategy aimed at screening closely related species. This approach holds promise in uncovering valuable compounds within their VOs, mirroring the success observed in the domain of medicinal plants.


The GC–MS characterization of O. syriacum VO indicated the presence of 22 components, with thymol, carvacrol, and γ-terpinene being the most prevalent. The most dominant components in C. serpyllifolium VO were p-cymene, carvacrol, and terpinene. Furthermore, 23 chemicals were discovered in T. capitata VO, with carvone and pulegone being the predominant components. The VO compositions were different from those found in other geographic areas. According to the antifungal activity results, all VOs from the three Palestinian Thyme species used in this investigation demonstrated strong action. The VOs isolated from O. syriacum and C. serpyllifolium displayed the strongest antifungal action against all clinical and ATCC Candida strains tested. Furthermore, in vivo, studies are necessary to validate the anticandidal activity of these VOs.

Availability of the data and materials

The datasets used and/or analyzed during the current study are available from the corresponding author upon reasonable request. The datasets supporting the conclusions of this article are included in the manuscript. The raw data and materials of the current study are available from the corresponding author upon reasonable request.


  1. Mesmar J, Abdallah R, Badran A, Maresca M, Baydoun E. Origanum syriacum phytochemistry and pharmacological properties: a comprehensive review. Molecules. 2022;27(13):4272.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  2. Amparo TR, Seibert JB, Silveira BM, Costa FSF, Almeida TC, Braga SFP, et al. Brazilian essential oils as source for the discovery of new anti-COVID-19 drug: a review guided by in silico study. Phytochem Rev. 2021;20:1–20.

    Article  Google Scholar 

  3. Cabral C, Efferth T, Pires IM, Severino P, Lemos MF. Natural products as a source for new leads in cancer research and treatment. Evidence-Based Complement Alter Med. 2018.

    Article  Google Scholar 

  4. Atanasov AG, Zotchev SB, Dirsch VM, Supuran CT. Natural products in drug discovery: advances and opportunities. Nat Rev Drug Discovery. 2021;20(3):200–16.

    Article  CAS  PubMed  Google Scholar 

  5. Anwar DM, El-Sayed M, Reda A, Fang J-Y, Khattab SN, Elzoghby AO. Recent advances in herbal combination nanomedicine for cancer delivery technology and therapeutic outcomes. Expert Opin Drug Delivery. 2021;11:1–17.

    Google Scholar 

  6. Shi S, Klotz U. Drug interactions with herbal medicines. Clin Pharmacokinet. 2012;51(2):77–104.

    Article  CAS  PubMed  Google Scholar 

  7. Wood DM, Athwal S, Panahloo A. The advantages and disadvantages of a “herbal” medicine in a patient with diabetes mellitus: a case report. Diabet Med. 2004;21(6):625–7.

    Article  CAS  PubMed  Google Scholar 

  8. Mahajan A, Kaur J, Kaur S. Herbal medicines: possible risks and benefits. Am J Phytomed Clin Ther. 2013;1:226–39.

    Google Scholar 

  9. El-Alam I, Zgheib R, Iriti M, El Beyrouthy M, Hattouny P, Verdin A, et al. Origanum syriacum essential oil chemical polymorphism according to soil type. Foods. 2019;8(3):90.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  10. Baytop T. Therapy with medicinal plants in Turkey (past and present). Turkey: Istanbul University; 1999.

    Google Scholar 

  11. Dunkić V, Kremer D, Grubešić RJ, Rodríguez JV, Ballian D, Bogunić F, et al. Micromorphological and phytochemical traits of four Clinopodium L species (Lamiaceae). South Afr J Bot. 2017;111:232–41.

    Article  Google Scholar 

  12. Gezici S, Koçum D, Yayla F, Sekeroglu N, Khan AA. Screening for in vitro antioxidant activities, polyphenolic contents and neuroprotective potentials of Clinopodium serpyllifolium subsp serpyllifolium endemic to Turkey. Ann Phytomed. 2020;9(1):181–6.

    Article  CAS  Google Scholar 

  13. Gagliano Candela R, Maggi F, Lazzara G, Rosselli S, Bruno M. The essential oil of Thymbra capitata and its application as a biocide on stone and derived surfaces. Plants. 2019;8(9):300.

    Article  PubMed  PubMed Central  Google Scholar 

  14. Verdeguer M, Torres-Pagan N, Muñoz M, Jouini A, García-Plasencia S, Chinchilla P, et al. Herbicidal activity of Thymbra capitata (L) Cav essential oil. Molecules. 2020;25(12):2832.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  15. Jackson BR, Chow N, Forsberg K, Litvintseva AP, Lockhart SR, Welsh R, et al. On the origins of a species: what might explain the rise of Candida auris? J Fungi. 2019;5(3):58.

    Article  Google Scholar 

  16. Ciurea CN, Kosovski I-B, Mare AD, Toma F, Pintea-Simon IA, Man A. Candida and candidiasis—opportunism versus pathogenicity: a review of the virulence traits. Microorganisms. 2020;8(6):857.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  17. Poulain D. Candida albicans, plasticity and pathogenesis. Crit Rev Microbiol. 2015;41(2):208–17.

    Article  CAS  PubMed  Google Scholar 

  18. Rodrigues CF, Rodrigues ME, Henriques M. Candida sp infections in patients with diabetes mellitus. J Clin Med. 2019;8(1):76.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Trofa D, Gácser A, Nosanchuk JD. Candida parapsilosis, an emerging fungal pathogen. Clin Microbiol Rev. 2008;21(4):606–25.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  20. Zuza-Alves DL, Silva-Rocha WP, Chaves GM. An update on Candida tropicalis based on basic and clinical approaches. Front Microbiol. 2017;8:1927.

    Article  PubMed  PubMed Central  Google Scholar 

  21. Fidel PL Jr, Vazquez JA, Sobel JD. Candida glabrata: review of epidemiology, pathogenesis, and clinical disease with comparison to C albicans. Clin Microbiol Rev. 1999;12(1):80–96.

    Article  PubMed  PubMed Central  Google Scholar 

  22. Benali T, Lemhadri A, Harboul K, Chtibi H, Khabbach A, Jadouali SM, et al. Chemical profiling and biological properties of essential oils of Lavandula stoechas L Collected from three Moroccan sites in vitro and in silico Investigations. Plants. 2023.

    Article  PubMed  PubMed Central  Google Scholar 

  23. Bellete B, Rabérin H, Flori P, El Akssi S, Tran Manh Sung R, Taourirte M, et al. Antifungal effect of the essential oil of Thymus broussonetii Boiss endogenous species of Morocco. Nat Prod Res. 2012;26(18):1692–6.

    Article  CAS  PubMed  Google Scholar 

  24. Giordani R, Regli P, Kaloustian J, Mikaïl C, Abou L, Portugal H. Antifungal effect of various essential oils against Candida albicans potentiation of antifungal action of amphotericin B by essential oil from Thymus vulgaris. Phytother Res. 2004;18(12):990–5.

    Article  CAS  PubMed  Google Scholar 

  25. Manohar V, Ingram C, Gray J, Talpur NA, Echard BW, Bagchi D, et al. Antifungal activities of origanum oil against Candida albicans. Mol Cell Biochem. 2001;228(1–2):111–7.

    Article  CAS  PubMed  Google Scholar 

  26. Potente G, Bonvicini F, Gentilomi GA, Antognoni F. Anti-candida activity of essential oils from Lamiaceae plants from the Mediterranean area and the Middle East. Antibiotics. 2020;9(7):395.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  27. Jaradat N, Adwan L, K’aibni S, Shraim N, An Z. Chemical composition, anthelmintic, antibacterial and antioxidant effects of Thymus bovei essential oil. BMC Complement Altern Med. 2016.

    Article  PubMed  PubMed Central  Google Scholar 

  28. Jaradat N. Quantitative estimations for the volatile oil by using hydro distillation and microwave accelerated distillation methods from Ruta graveolens L and Ruta chalepensis L leaves from Jerusalem area/Palestine. Moroccan J Chem. 2016;4(1):4–1.

    Google Scholar 

  29. Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal. 2016;6(2):71–9.

    Article  PubMed  Google Scholar 

  30. Qadi M, Jaradat N, Al-lahham S, Ali I, Abualhasan MN, Shraim N, et al. Antibacterial, anticandidal, phytochemical, and biological evaluations of pellitory plant. Biomed Res Int. 2020;2020:6965306.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  31. Al Hafi M, El Beyrouthy M, Ouaini N, Stien D, Rutledge D, Chaillou S. Chemical composition and antimicrobial activity of origanum libanoticum, origanum ehrenbergii, and origanum syriacum growing wild in lebanon. Chem Biodivers. 2016;13(5):555–60.

    Article  CAS  PubMed  Google Scholar 

  32. Braga PC, Culici M, Alfieri M, Dal Sasso M. Thymol inhibits Candida albicans biofilm formation and mature biofilm. Int J Antimicrob Agents. 2008;31(5):472–7.

    Article  CAS  PubMed  Google Scholar 

  33. de Castro RD, de Souza TM, Bezerra LM, Ferreira GL, Costa EM, Cavalcanti AL. Antifungal activity and mode of action of thymol and its synergism with nystatin against Candida species involved with infections in the oral cavity: an in vitro study. BMC Complement Altern Med. 2015;15:417.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  34. Houst J, Spizek J, Havlicek V. Antifungal drugs. Metabolites. 2020;10:106.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  35. Khan MSA, Malik A, Ahmad I. Anti-candidal activity of essential oils alone and in combination with amphotericin B or fluconazole against multi-drug resistant isolates of Candida albicans. Med Mycol. 2012;50(1):33–42.

    Article  CAS  PubMed  Google Scholar 

  36. Abdalrazeq M, Jaradat N, Qadi M, Giosafatto CVL, Dell’Olmo E, Gaglione R, et al. Physicochemical and antimicrobial properties of whey protein-based films functionalized with palestinian satureja capitata essential oil. Coatings. 2021;11(11):1364.

    Article  CAS  Google Scholar 

  37. Baydar H, Sağdiç O, Özkan G, Karadoğan T. Antibacterial activity and composition of essential oils from Origanum, Thymbra and Satureja species with commercial importance in Turkey. Food Control. 2004;15(3):169–72.

    Article  CAS  Google Scholar 

  38. Gholami-Ahangaran M, Ahmadi-Dastgerdi A, Azizi S, Basiratpour A, Zokaei M, Derakhshan M. Thymol and carvacrol supplementation in poultry health and performance. Veterinary Med Sci. 2022;8(1):267–88.

    Article  CAS  Google Scholar 

  39. Balahbib A, El Omari N, Hachlafi NE, Lakhdar F, El Menyiy N, Salhi N, et al. Health beneficial and pharmacological properties of p-cymene. Food Chem Toxicol. 2021;153: 112259.

    Article  CAS  PubMed  Google Scholar 

  40. Leyva-López N, Gutiérrez-Grijalva EP, Vazquez-Olivo G, Heredia JB. Essential oils of oregano: Biological activity beyond their antimicrobial properties. Molecules. 2017;22(6):989.

    Article  PubMed  PubMed Central  Google Scholar 

  41. Tariq S, Wani S, Rasool W, Shafi K, Bhat MA, Prabhakar A, et al. A comprehensive review of the antibacterial, antifungal and antiviral potential of essential oils and their chemical constituents against drug-resistant microbial pathogens. Microb Pathog. 2019;134: 103580.

    Article  CAS  PubMed  Google Scholar 

  42. Salameh N, Shraim N, Jaradat N, El Masri M, Adwan L, K’aibni S, et al. Screening of antioxidant and antimicrobial activity of Micromeria fruticosa serpyllifolia volatile oils: a comparative study of plants collected from different regions of west Bank Palestine. BioMed Res Int. 2020.

    Article  PubMed  PubMed Central  Google Scholar 

  43. Karpiński TM. Essential oils of lamiaceae family plants as antifungals. Biomolecules. 2020;10(1):103.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  44. Gelmini F, Squillace P, Testa C, Sparacino A, Angioletti S, Beretta G. GC–MS characterisation and biological activity of essential oils from different vegetative organs of Plectranthus barbatus and Plectranthus caninus cultivated in north Italy. Nat Prod Res. 2015;29(11):993–8.

    Article  CAS  PubMed  Google Scholar 

  45. Ghasemi E, Sharafzadeh S, Amiri B, Alizadeh A, Bazrafshan F. Variation in essential oil constituents and antimicrobial activity of the flowering aerial parts of Salvia mirzayanii Rech Esfand Ecotypes as a folkloric herbal remedy in Southwestern Iran. J Essential Oil Bearing Plants. 2020;23(1):51–64.

    Article  CAS  Google Scholar 

  46. Cox S, Mann C, Markham J, Bell HC, Gustafson J, Warmington J, et al. The mode of antimicrobial action of the essential oil of Melaleuca alternifolia (tea tree oil). J Appl Microbiol. 2000;88(1):170–5.

    Article  CAS  PubMed  Google Scholar 

  47. Zengin H, Baysal AH. Antibacterial and antioxidant activity of essential oil terpenes against pathogenic and spoilage-forming bacteria and cell structure-activity relationships evaluated by SEM microscopy. Molecules. 2014;19(11):17773–98.

    Article  PubMed  PubMed Central  Google Scholar 

  48. Hammer K, Carson C, Riley T. Antifungal activity of the components of Melaleuca alternifolia (tea tree) oil. J Appl Microbiol. 2003;95(4):853–60.

    Article  CAS  PubMed  Google Scholar 

  49. Sikkema J, de Bont JA, Poolman B. Mechanisms of membrane toxicity of hydrocarbons. Microbiol Rev. 1995;59(2):201–22.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

Download references


The authors would like to acknowledge the faculty of Medicine and Health Sciences at An-Najah National University.


Not funded.

Author information

Authors and Affiliations



The current research done by the authors for submission.

Corresponding author

Correspondence to Mohammad Qadi.

Ethics declarations

Ethics approval and consent to participate

Not applicable.

Consent for publication

The authors of the current work gave consent for publication to Dr. Mohammad Qadi.

Competing interests

The authors declare that they have no competing interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit The Creative Commons Public Domain Dedication waiver ( applies to the data made available in this article, unless otherwise stated in a credit line to the data.

Reprints and Permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Qadi, M., Jaradat, N., Al-Maharik, N. et al. Anticandidal effects and chemical compositions of volatile oils extracted from Origanum syriacum, Clinopodium serpyllifolium subsp. fruticosum and Thymbra capitata from Palestine. Chem. Biol. Technol. Agric. 10, 87 (2023).

Download citation

  • Received:

  • Accepted:

  • Published:

  • DOI: