Physiological responses to humic substances as plant growth promoter
© Canellas and Olivares; licensee Springer. 2014
Received: 28 October 2013
Accepted: 10 January 2014
Published: 13 March 2014
Humic substances (HS) have been widely recognized as a plant growth promoter mainly by changes on root architecture and growth dynamics, which result in increased root size, branching and/or greater density of root hair with larger surface area. Stimulation of the H+-ATPase activity in cell membrane suggests that modifications brought about by HS are not only restricted to root structure, but are also extended to the major biochemical pathways since the driving force for most nutrient uptake is the electrochemical gradient across the plasma membrane. Changes on root exudation profile, as well as primary and secondary metabolism were also observed, though strongly dependent on environment conditions, type of plant and its ontogeny. Proteomics and genomic approaches with diverse plant species subjected to HS treatment had often shown controversial patterns of protein and gene expression. This is a clear indication that HS effects of plants are complex and involve non-linear, cross-interrelated and dynamic processes that need be treated with an interdisciplinary view. Being the humic associations recalcitrant to microbiological attack, their use as vehicle to introduce beneficial selected microorganisms to crops has been proposed. This represents a perspective for a sort of new biofertilizer designed for a sustainable agriculture, whereby plants treated with HS become more susceptible to interact with bioinoculants, while HS may concomitantly modify the structure/activity of the microbial community in the rhizosphere compartment. An enhanced knowledge of the effects on plants physiology and biochemistry and interaction with rhizosphere and endophytic microbes should lead to achieve increased crop productivity through a better use of HS inputs in Agriculture.
Natural organic matter (NOM) is a product of plant and animal tissue decay, and, together with the biota, is of pivotal importance in the global carbon cycle. The relationship between NOM and crop production is consistent with the results of over a hundred years of modern agricultural research and with thousands of years of on-farm observation and experimentation . This relationship is based on the critical influence of NOM on soil properties, processes and functions. Humus, otherwise referred to as HS, is the NOM comprising up to 80% of soil organic matter. Because of the beneficial effects that HS have on the physical, chemical, and biological properties of soil, their role in sustaining plant growth is recognized.
The direct use of soluble HS as a plant growth promoter is not a novelty, but the increase of HS market as plant stimulators has attracted the attention of new producers, businesses and farmers interested in knowing how such dilute concentrations can bring many benefits to sustainable production. The most described effect of HS is the promotion of plant root system. However, primary plant metabolism, diverse and complex enzymatic machinery related with a plethora of cell processes and more recent changes on secondary metabolism by HS are being increasingly documented.
The root system plays a central role in the acquisition of water and nutrients in a natural heterogeneous soil environment and how plants promote changes in its rhizosphere for defence, improve nutrient mineralization and select microbial community. These processes could be modulated by HS. It is not a surprise that HS can modify plant root growth and architecture since the genesis and soil use history is profoundly marked by HS formation. During the conquest of land, the salt stress ceased to exist, and plants switched to H+ as the master ion . Brundrett ) suggests that, as plants colonized the land, they would have faced powerful selection pressure to increase the surface area of their absorptive surfaces in soil to parallel that occurring in their photosynthetic organs. Interception of light and CO2 fixation would have thereby become in balance with nutrients and water uptake from soils. How HS pressed this selection is not very clear but its influence is presumable.
Recent advances of knowledge on humus chemistry have emphasize that small and heterogeneous molecules are randomly associated in hydrophobic-hydrophilic phases which are either contiguous to or contained in each other . Their environmental reactivity is dictated by their hydophobic/hydrophilic ratio  and a detailed molecular characterization of HS would eventually allow a structure-activity relationship between humic molecules and their activity inside plant cells [12–14]. Manufactured technologies for controlling HS activity can now take a boost. Powerful methods on molecular biology along with new innovative methods for conservation of agricultural diversity demand a new generation of agrochemicals acting as plant growth promoters based on the physiological effects of natural humic matter. The objective to be achieved is the improvement of crop productivity by relating structure and conformation of HS to plant physiological and biochemical activity.
Here we consider some of the main mechanisms involved in root growth promotion, absorption of water and nutrients, and interaction with beneficial soil microbes mediated by soluble HS applied directly to plants at low concentration. The implications on primary and secondary plant metabolism will be also briefly discussed.
Changes in root architecture and morphology by HS
The elongation and differentiation zone of roots include small, dense meristematic cells that are in continuous metabolic activity and are susceptible to lateral root formation. HS were found to have marked effect on the emergence of lateral roots and the hyperinduction of sites of lateral root emergence upon HS treatments have been observed (Figure 2B; [15, 16]).
Quaggiotti et al.  provided unequivocal evidence of the expression induced by HS from the major isoform of the maize plasma membrane H+-ATPase (MHA2), which was characterized by Frias et al. . An about two-fold increase of mRNA levels of this isoform was observed in seedlings treated with HS. The HS-induced synthesis of new H+-ATPases enhanced not only H+-extrusion, but also the number of H+-ATPase in a given membrane area. Despite the extension of plasma membrane during growth, these changes resulted in the maintenance of the membrane potential, the energy-dependent transport of solutes to and from the cell and the turgor . Zandonadi et al.  showed that different HS isolated from different sources as well as low auxin concentrations (10−10 and 10−15 M) promoted a differential activation not only of the plasma membrane but also of vacuolar H+-ATPases and H+-pyrophosphatase. This represents a complementary view of the acid growth mechanism in which a concerted activation of the plasmalemma and tonoplast H+ pumps plays a key role in the root cell expansion process, which is driven by environment-derived molecules endowed with auxin activity, such as HS. On the other hand, activation of PM H+-ATPase also improves plant nutrition by enhancing the electrochemical proton gradient that drives ion transport across cell membranes via secondary transport systems . It is not surprising to find nutrient accumulation in plants treated with HS as observed by Baldotto et al.  in pineapples, whereby the increase of N, P, K, Ca and Mg in leaves was 52%, 71%, 50%, 58% and 59% larger than control, respectively.
Zandonadi et al.  showed that HS can induce nitric oxide (NO) in sites of lateral root emergence. NO is a bioactive molecule that is involved in numerous plant physiological processes including root development among others . NO was also shown to be involved in Arabidopsis root hair formation in both initiation and elongation processes [31, 32]. The application of HS on roots of cucumber plants caused a primary increase in NO accumulation and it was associated by Mora et al.  with the expression of the following morphological root changes: (1) increase in the number of secondary roots that were measured in the medium region of root system, (2) increase in root thickness, and (3) increase in root fresh weight.
The disruption of HS associations in solution by the action of organic acids such as those exuded by plants and soil biomass has been repeatedly indicated . It is thus conceivable that molecules released from humic superstructures may then access cell membranes and induce different physiological responses. The assembling and disassembling behavior of HA molecular domains and their dynamic release of different molecular constituents  may then explain why humic compounds had been previously found to possess hormone-like activities similar to those of auxins, gibberellins, cytokinins and polyamines . The ultimate physiological response would involve a large level of cell activity and tissue differentiation, which result in root growth.
Changes on primary and secondary metabolism
Primary metabolism refers to a biochemical basic process essential for plant survival, growth and reproduction. The involved molecules are called primary metabolites and are generally constituted by proteins, carbohydrates and lipids. Glycolysis is a basic metabolic process with crucial importance in plants because it is the predominant pathway that ‘fuels’ plant respiration. Nardi et al.  showed that HS affected the enzyme activities related to glycolysis and the tricarboxylic acid cycle (TCA) in different ways, depending on humic molecular size, molecular characteristic and concentration. The glycolysis enzymatic activities studied by Nardi et al.  were glucokinase, phosphoglucose isomerase, PPi-dependent phosphofructokinase and pyruvate kinase, while those involved in the respiration process were cytrate synthase, malate dehydrogenase and the cytosolic form of NADP+-isocitrate dehydrogenase. The stimulation increased significantly for all enzymes at 1 mg C · L−1 for either a soil HS or its fraction separated by size exclusion chromatography. It was also found as a positive effect of HS on the main photosynthetic metabolism in maize leaves, where a decrease in starch content was accompanied by an increase in soluble sugars . Moreover, sucrose, which is synthesized from carbohydrates produced by photosynthesis via the reductive pentose phosphate (Calvin-Benson) pathway, represents the starting compound for the respiratory pathway, and its increase may justify the 40% activity improvement of rubisco (d-ribulose-1,5-bisphosphate carboxylase/oxygenase activity) in plants treated with leonardite HS . This change appeared to be mediated by variations in the activity of the main enzymes involved in carbohydrate metabolism. According to Merlo et al. , in most plants, starch and sucrose are the principal end products of photosynthesis. The cytoplasmatic fructose-1,6-bisphosphate and sucrose phosphate synthase appear to be important enzymes in the control of sucrose formation, whereas ADP-glucose pyrophosphorylase has a key role in the starch biosynthesis pathway. These authors found that leaf starch content decreased in plants treated with HS, whereas the level of soluble sugars concomitantly increased. The decrease of starch was accompanied by an enhanced activity of amylase, whereas the activity of ADP glucose pyrophosphorylase was not affected. Activities of invertases and sucrose synthase were stimulated by HS treatment in apical tissues and inhibited when HS were applied to basal tissues. HS may change both the level and percent distribution of sugars of maize leaves, by affecting enzyme activities involved in carbohydrate metabolism. More recently, Canellas et al.  verified that free carbohydrate content in leaf extracts was 60% lower than control in maize plants treated with HS, showing reduction of fructose, glucose and starch. Finally, the induction of leaf invertase activity by HS was also verified by Concheri et al.  in wheat root seedlings.
The N metabolism is the basis of amino acids, proteins, enzymes and nucleotide formation. The enhancement of N uptake/assimilation and N metabolism in plants treated with HS had been well documented. Piccolo et al.  showed that HS increased uptake by barley seedlings to the same extent previously observed by Albuzio et al. . Quaggiotti et al.  in a seminal experiment did not find a relationship between uptake and high/or low affinity of cell transporter to HS stimuli. This appears to confirm the hypothesis proposed by Nardi et al.  that stimulation of nitrate uptake by HS cannot be explained by an effect on the primary transport of solutes, but it should instead decrease pH at the root surface, thus facilitating the H+/ symport. As cited earlier, Quaggiotti et al.  elegantly demonstrated the induction of MHA2 plasma membrane H+-ATPase isoform by HS. Besides uptake, HS also induce changes on N cell metabolism. For example, Ertani et al.  evaluated, in maize treated by HS, the activities of glutamine synthetase (GS EC 184.108.40.206) and glutamate synthase (GOGAT EC 220.127.116.11), which are key enzymes involved in N assimilation. They found a 65% increase in GS root activities and GOGAT enzyme activity increased by 176% in the roots and 204% in leaves, in comparison to control. The influence of soil HS on uptake was studied by Panuccio et al.  in two different coniferous species. They measured the activity of key enzymes also involved in N metabolism, such as malate dehydrogenase (MDH; EC 18.104.22.168), glutamate dehydrogenase (GDH; EC 22.214.171.124) and phosphoenolpyruvate carboxylase (PEPC; EC 126.96.36.199), and observed enzyme activities increased concomitantly with ammonium uptake but not in nitrate uptake.
These results revealed the role of HS in primary plant metabolism and in enzyme activity modulation. Carletti et al.  reported for the first time a proteomic approach in a study on maize seedlings treated with HS. These authors isolated plasma membrane proteins, including triosophosphate isomerase (TIM; EC 188.8.131.52), glyceraldehyde-3-P dehydrogenase (GAPD; EC 184.108.40.206), phosphoglycerate mutase (PGAM; EC 220.127.116.11), fructose biphosphate aldolase (FBA; EC 18.104.22.168), 2-phosphoglycerate dehydratase (enolase, ENO; EC 22.214.171.124), phosphoglucomutase (PGM; EC 126.96.36.199), and 3-phosphoglycerate kinase (PGK; EC 188.8.131.52), and verified whether these were down-regulated by the HS treatment. They found that the isolated proteins were all down-regulated by the HS treatment, except for TIM, which showed a 69% increase, and GAPD, which maintained the same level of expression in treated and untreated samples. Moreover, they identified two adjacent protein spots as cytosolic aconitate hydratase (ACO), one showing down regulation (spot 230, −77%) and the other up regulation (spot 224, +79%). This result is suggestive of a post-translational modification of the enzyme. The expression of ACO may induce changes in root excretion and participate in the rhizosphere cross-talk between plant and soil. This was observed by Canellas et al.  and Puglisi et al. , who showed an increase in acid citric exudation by maize seedlings when treated with different HS. Citric acid is a component of root exudates and plays an important role in disaggregating humic supramolecular structures .
The proteomic approach used to assess the effect of HS on plant metabolism was followed by a genomic assay in Arabidopsis thaliana treated with HS . Down and up regulation behaviours were also observed, thereby demonstrating that HS affect plant physiology by means of complex transcriptional networks. From the overall transcriptomic results, we may hypothesize that HS exert their function through a multifaceted mechanism of action, partially connected to their well-demonstrated auxin activity, but involving also IAA-independent signalling pathways.
The Pandora box was open and elucidation of complex interactions between plant and HS should be done step by step and should grant more reliability on the recommendation of doses and times of applications to obtain plant growth stimulation based on HS physiological effects. For instance, Jannin et al.  used microarray analysis to evaluate genes involved in physiological response of Brassica napus to HS and indentified over expression in nine clusters, which covered the major metabolic plant functions: carbon and photosynthesis, general cell metabolism, fatty acids, nitrogen/sulphur, phytohormones, plant development, senescence, responses to stress and transport of ions and water. HS can thus induce carbon and nitrogen metabolism and a plethora of cell process can be down or up regulated .
Schiavon et al.  showed for the first time a relevant effect of HS on secondary plant metabolism. Plants produce a remarkably diverse array of over 100,000 low-molecular-mass natural products, also known as secondary metabolites that are distinct from the components of primary metabolism since they are generally non-essential for the basic metabolic processes. According to Dixon , most of the secondary metabolites are derived from the isoprenoid, phenylpropanoid, alkaloid or fatty/polyketide pathways and are produced to improve plant defence against biotic and abiotic stress. Schiavon et al.  showed that HS treatment enhanced the expression of the phenylalanine (tyrosine) ammonia-lyase (PAL/TAL; EC 184.108.40.206) that catalyzes the first committed step in the biosynthesis of phenolics, by converting phenylalanine to trans-cinnamic acid and tyrosine to p-coumaric acid. The expression of PAL/TAL was accompanied by leaf phenol accumulation. The authors concluded that the stimulatory effects of HS on plant secondary metabolism provide an innovative approach to explore plant responses to stress. The influence of HS on enzyme activities linked to cell protection has been observed and explained as induction of esterase activity  and protection against oxidative stress . The door was open to drive the use of HS as stimulator of secondary metabolites in improving medicinal, aromatics, spices plants and reducing the use of synthetic agrochemical as plant protector.
HS and plant growth-promoting bacteria
By definition, HS are assemblies of heterogeneous compounds that are insoluble in water and recalcitrant to microbial activity and represent the stable part of earth C cycle . However, since HS affect both plant primary and secondary metabolism including changes on exudation profile, it is pertinent to consider that HS may interfere with microorganism community in the rhizosphere. Puglisi et al. ; ) showed that the addition of HS had a significant effect on the amount of bioavailable C deposited by maize plant roots, thus resulting in a significant change in the structure of soil microbial communities. More recently, Puglisi et al.  used DGGE analysis and showed that the influence of HS on microorganism diversity reaches the bulk soil beyond the rhizosphere zone. Plants thus select their microbial community in order to improve their physiological processes involved in defence against pathogens, and mineralization and solubilization of nutrients.
Plant growth-promoting bacteria (PGPB) are a wide range of microorganisms that induce plant growth by several processes including biological N2 fixation, increase of nutrient availability in the rhizosphere, enlargement of root surface area, and enhancement of beneficial symbioses for the host . Relationships between PGPB and their hosts can be either rhizospheric or endophytic. We proposed a new biofertilizer concept based on the combination of HS and endophytic diazotrophic bacteria. Such biofertilizer implies an increase of endophytic interactions associated with plant host and protection of the bioinoculant in the HS hydrophobic domains which act as carriers. Different from rhizobia-legume symbioses, endophytic PGPB interaction do not have specialized root hair infection mechanisms, which are induced by chemotaxis responses to flavonoid compounds present in legume exudates . The infection of endophytic PGPB is opportunistic since it exploits the natural openings occurring during the lateral root emergence that is significantly promoted by HS. The result is an increased population of endophytic bacteria associated to plants. Furthermore, highly efficient PGPB are selected in the laboratory by allowing maximum expression of their beneficial traits such as auxin (and other hormones) production, phosphate solubilization, antibiosis, etc. However, the introduction of selected PGPB to crop field represents a surviving challenge for incoming microorganisms which have to face the competition with natural microbiota adapted to infection niches in the host rhizosphere.
The anatomical changes deriving from an increase of both area and length of root hair favour bacteria association with root surface. The mechanism of endophytic penetration of bacteria is crucial for their activity in plants  and represents the selective advantage for bacteria competing for colonization sites in plants. The bacterial population with largest attachment to roots surfaces is more likely to develop biofilms, thereby increasing their activity, persistency and overall probability to colonize the inner tissue of the inoculated plant (Figure 7). The observations of such structural interactions between bacteria and plants in the presence of HS represent the explanation for the positive and consistent effects of the combined use of humic acids and PGPB under field conditions.
Field experiments using low fertilizer input (20 kg N-urea) and foliar application of both HS from vermicompost and a plant growth-promoting bacteria strain of H. seropedicae (Z67) were carried out . It was found, at early stages (7 and 45 days old) in greenhouse, that H. seropedicae in mixture with HS from vermicompost activated plant metabolic processes such as enhancement of plasma membrane H+-ATPase activity, alteration of both sugar and N metabolism and increase of net photosynthesis. The number of viable bacterial cells was larger in root tissues when inoculation was performed in the presence of soluble HS. Foliar application of endophytic diazotrophic bacteria in mixture with HS increased maize grain production by 65% under field conditions. Again in field experiments, a 30% yield increase of sugarcane stems was obtained by foliar application of H. seropedicae in mixture with humic acid (Figure 9), without any negative effect for the agroindustrial sugar content (data not shown).
Zaler  proposed the use of vermicompost as suitable plant growth media in horticulture instead of peat, whose indiscriminate use menaces endangered bog ecosystems worldwide. The benefits of vermicompost to field crops are well documented  and are often attributed to its large nutrient potential, content of humified molecules possessing hormone-like activity , and presence of bioactive microorganisms. The introduction of a mixed inoculum of PGPB strains of H. seropedicae (HRC 54) and Burkholderia silvatlantica (UENF 101) at the initial stage of the vermicomposting process revealed the increase of total N and water-soluble phosphorus in the mature vermicompost , thus showing that the addition of beneficial microorganisms enables the strengthening of the growth substrate. Moreover, in a study on the organic matter molecular composition of different vermicomposts, a significant relationship was found between the presence of long-chain hydrophobic compounds in compost and the preservation of PGPB during 1 year after inoculation .
Humic substances (HS) are the major fraction of the soil organic matter which represent the final stage of a complex interaction between non-living organic matter and microbial communities. The critical influence of HS on the physical, chemical, and biological properties of soil has been extensively demonstrated and their role in sustaining plant growth recognized. More recently, a number of experimental data has shown that HS and its different fractions may affect plant growth and development, involving specific structural and physiological responses to HS applications. Remarkable effects on root architecture, such as induction of lateral root and root hair may be accompanied by changes in the biochemistry of energy generation and transport system across plasma membranes. Primary and secondary plant metabolisms of above- and below-ground tissues are recognized to be affected by HS. Thus, a systematic and coordinated research combining different approaches in functional genomics (e.g. transcriptomic and metabolomic) may help to overcome the complexity if HS influence on plant biology, and allow developing new technologies to increase plant growth based on humic matter. Technological research have already shown the benefits of combining the application of plant growth-promoting microbes with humic substances, the latter acting as a transporter for microbial delivery under field conditions. This synergy of effects favours the increase in population and activity of selected microbes and consequent response to plant growth promotion, thus opening opportunities to develop a new generation of biofertilizers for sustainable agricultural systems.
The work in NUDIBA laboratory was supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Fundação de Amparo à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Instituto Nacional de Ciência e Tecnologia (INCT) para a Fixação Biológica de Nitrogênio, Internacional Foundation of Science (IFS) and OCWP. We are also grateful to editor and three anonymous reviewers for their constructive comments on the original version of the manuscript, which helped to improve it greatly. Lastly, we apologize to those authors whose work could not be discussed because of the space limitation.
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